ArticleMolecular and cellular biology2001
The gypsy insulator can act as a promoter-specific transcriptional stimulator.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- The Role of Insulators in Transgene Transvection inGenetics · 2019Article
- Transposon-mediated rewiring of gene regulatory networks contributed to the evolution of pregnancy in mammals.Nature genetics · 2011Article
- Evidences for insulator activity of the 5'UTR of the Drosophila melanogaster LTR-retrotransposon ZAM.Molecular genetics and genomics : MGG · 2010Article
- Study of long-distance functional interactions between Su(Hw) insulators that can regulate enhancer-promoter communication in Drosophila melanogaster.Molecular and cellular biology · 2006Article
- Pairing between gypsy insulators facilitates the enhancer action in trans throughout the Drosophila genome.Molecular and cellular biology · 2005Article
- Drosophila Su(Hw) insulator can stimulate transcription of a weakened yellow promoter over a distance.Genetics · 2005Article
- The Mod(mdg4) component of the Su(Hw) insulator inserted in the P transposon can repress its mobility in Drosophila melanogaster.Genetics · 2004Article
- An endogenous suppressor of hairy-wing insulator separates regulatory domains in Drosophila.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Functional dissection of the mouse tyrosinase locus control region identifies a new putative boundary activity.Nucleic acids research · 2003Article
- Rationally designed insulator-like elements can block enhancer action in vitro.The EMBO journal · 2003Article
- Spatial organization of gene expression: the active chromatin hub.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Review
- Analysis of the effect of the Su(Hw) insulator on the expression of the miniwhite gene in Drosophila melanogaster.Doklady. Biochemistry and biophysicsArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insulators define chromosomal domains such that an enhancer in one domain cannot activate a promoter in a different domain. We show that the Drosophila gypsy insulator behaves as a cis-stimulatory element in the larval fat body. Transcriptional stimulation by the insulator is distance dependent, as expected for a promoter element as opposed to an enhancer. Stimulation of a test alcohol dehydrogenase promoter requires a binding site for a GATA transcription factor, suggesting that the insulator may be facilitating access of this DNA binding protein to the promoter. Short-range stimulation requires both the Suppressor of Hairy-wing protein and the Mod(mdg4)-62.7 protein encoded by the trithorax group gene mod(mdg4). In the absence of interaction with Mod(mdg4)-62.7, the insulator is converted into a short-range transcriptional repressor but retains some cis-stimulatory activity over longer distances. These results indicate that insulator and promoter sequences share important characteristics and are not entirely distinct. We propose that the gypsy insulator can function as a promoter element and may be analogous to promoter-proximal regulatory modules that integrate input from multiple distal enhancer sequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.