ArticleNucleic acids research2001
In vivo gene repair of point and frameshift mutations directed by chimeric RNA/DNA oligonucleotides and modified single-stranded oligonucleotides.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 92 citations in OpenAlex.
- Emerging gene editing strategies for Duchenne muscular dystrophy targeting stem cells.Frontiers in physiology · 2014Review
- Zinc finger nucleases for targeted mutagenesis and repair of the sickle-cell disease mutation: An in-silico study.BMC blood disorders · 2012Article
- Single-strand DNA-mediated targeted mutagenesis of genomic DNA in early mouse embryos is stimulated by Rad51/54 and by Ku70/86 inhibition.Gene therapy · 2008Article
- Single-stranded oligonucleotide-mediated in vivo gene repair in the rd1 retina.Molecular vision · 2007Article
- Intracellular distribution of TiO2-DNA oligonucleotide nanoconjugates directed to nucleolus and mitochondria indicates sequence specificity.Nano letters · 2007Article
- Manipulation of cell cycle progression can counteract the apparent loss of correction frequency following oligonucleotide-directed gene repair.BMC molecular biology · 2007Article
- Article
- Reporter system for the detection of in vivo gene conversion: changing colors from blue to green using GFP variants.Molecular biotechnology · 2006Article
- Oligonucleotide-directed gene repair in wheat using a transient plasmid gene repair assay system.Plant cell reports · 2006Article
- Generation of a mouse mutant by oligonucleotide-mediated gene modification in ES cells.Nucleic acids research · 2006Article
- Site-specific base changes in the coding or promoter region of the human beta- and gamma-globin genes by single-stranded oligonucleotides.The Biochemical journal · 2005Article
- Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis.Nucleic acids research · 2005Article
- Reaction parameters of targeted gene repair in mammalian cells.Molecular biotechnology · 2005Article
- Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Site-specific strand bias in gene correction using single-stranded oligonucleotides.Journal of molecular medicine (Berlin, Germany) · 2005Article
- Correction of the neuropathogenic human apolipoprotein E4 (APOE4) gene to APOE3 in vitro using synthetic RNA/DNA oligonucleotides (chimeraplasts).Journal of molecular neuroscience : MN · 2005Article
- Targeted conversion of the transthyretin gene in vitro and in vivo.Gene therapy · 2004Article
- Camptothecin enhances the frequency of oligonucleotide-directed gene repair in mammalian cells by inducing DNA damage and activating homologous recombination.Nucleic acids research · 2004Article
- Genetic re-engineering of Saccharomyces cerevisiae RAD51 leads to a significant increase in the frequency of gene repair in vivo.Nucleic acids research · 2004Article
- Fluorescent detection and isolation of DNA variants using stabilized RecA-coated oligonucleotides.Genome research · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Synthetic oligonucleotides have been used to direct base exchange and gene repair in a variety of organisms. Among the most promising vectors is chimeric oligonucleotide (CO), a double-stranded, RNA-DNA hybrid molecule folded into a double hairpin conformation: by using the cell's DNA repair machinery, the CO directs nucleotide exchange as episomal and chromosomal DNA. Systematic dissection of the CO revealed that the region of contiguous DNA bases was the active component in the repair process, especially when the single-stranded ends were protected against nuclease attack. Here, the utility of this vector is expanded into Saccharomyces cerevisiae. An episome containing a mutated fusion gene encoding hygromycin resistance and eGFP expression was used as the target for repair. Substitution, deletion and insertion mutations were corrected with different frequencies by the same modified single-stranded vector as judged by growth in the presence of hygromycin and eGFP expression. A substitution mutation was repaired the most efficiently followed by insertion and finally deletion mutants. A strand bias for gene repair was also observed; vectors designed to direct the repair of nucleotide on the non-transcribed (non-template) strand displayed a 5-10-fold higher level of activity. Expanding the length of the oligo-vector from 25 to 100 nucleotides increases targeting frequency up to a maximal level and then it decreases. These results, obtained in a genetically tractable organism, contribute to the elucidation of the mechanism of targeted gene repair.
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Registered trials
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