ArticleGut2001
Interleukin 2 modulates ion secretion and cell proliferation in cultured human small intestinal enterocytes.
Article in Gut, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Two Different Copy Number Variations of theAnimals : an open access journal from MDPI · 2021Article
- PK15 cell line stably overexpressing IL2 enhances PCV2 replication.Virus genes · 2021Article
- Plasma Rich in Growth Factors (PRGF) Increases the Number of Retinal Müller Glia in Culture but Not the Survival of Retinal Neurons.Frontiers in pharmacology · 2021Article
- Low-dose interleukin-2 alleviates dextran sodium sulfate-induced colitis in mice by recovering intestinal integrity and inhibiting AKT-dependent pathways.Theranostics · 2020Article
- Goblet Cell Derived RELM-β Recruits CD4+ T Cells during Infectious Colitis to Promote Protective Intestinal Epithelial Cell Proliferation.PLoS pathogens · 2015Article
- Interleukin-21 receptor signalling is important for innate immune protection against HSV-2 infections.PloS one · 2013Article
- Molecular mechanism of interleukin-2-induced mucosal homeostasis.American journal of physiology. Cell physiology · 2012Article
- Ion channels in inflammation.Pflugers Archiv : European journal of physiology · 2011Review
- The IL-2/CD25 pathway determines susceptibility to T1D in humans and NOD mice.Journal of clinical immunology · 2008Article
- Involvement of chloride channels in IGF-I-induced proliferation of porcine arterial smooth muscle cells.Cardiovascular research · 2007Article
- Changes in ion transport in inflammatory disease.Journal of inflammation (London, England) · 2006Article
- Modulation of human enteric epithelial barrier and ion transport function by Peyer's patch lymphocytes.World journal of gastroenterology · 2004Article
- Inhibition of small-conductance Cl- channels by the interleukin-1beta-stimulated production of superoxide in rabbit gastric parietal cells.The Journal of physiology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo determine if interleukin 2 (IL-2) alters epithelial transport and barrier function in cultured human small intestinal enterocytes.
methodsConfluent monolayers of small intestinal cells derived from duodenal biopsies were treated with IL-2 0.2-50 U/ml for 24 hours prior to study. Transport measurements were performed under short circuited conditions in Ussing chambers, with and without the secretagogues forskolin and 3-isobutyl-1-methyl xanthine (IBMX). Serosal to mucosal flux of 3[H] mannitol (permeability) and 3[H] thymidine uptake (proliferation) were measured. IL-2 receptor and cystic fibrosis transmembrane conductance regulator (CFTR) mRNA were identified using reverse transcription-polymerase chain reaction (RT-PCR).
resultsIL-2 did not alter baseline electrical parameters but caused a significant increase in cAMP dependent chloride secretion. The effect was mediated by the IL-2 receptor and paralleled a rapid increase in tyrosine phosphorylation, janus kinase 1, and signal transducers and activators of transcription (STATs) 1, 3, and 5. IL-2 significantly increased proliferation but at a lower dose than observed for enhanced secretion but did not alter permeability. IL-2 receptor beta and gammac chains and CFTR mRNA were identified by RT-PCR.
conclusionsIL-2 treatment enhances cAMP stimulated chloride secretion and cellular proliferation in a human small intestinal cell line expressing a functional IL-2 receptor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.