ArticlePlant physiology2001
Analysis of the compartmentation of glycolytic intermediates, nucleotides, sugars, organic acids, amino acids, and sugar alcohols in potato tubers using a nonaqueous fractionation method.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 257 citations in OpenAlex.
- PERSPECTIVE: organelle positioning as a principle of metabolic regulation and stress tolerance.The Plant journal : for cell and molecular biology · 2026Review
- Measurement of Subcellular Metabolite Concentrations in Relation to Phloem Loading.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Glutamine alleviates the toxicity of externally applied amino acids in Arabidopsis.Frontiers in plant science · 2026Article
- Gas Chromatography-Mass Spectrometry (GC-MS) in the Plant Metabolomics Toolbox: Sample Preparation and Instrumental Analysis.Biomolecules · 2025Review
- Review
- The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole.Metabolites · 2021Article
- Sucrose Metabolism and Transport in Grapevines, with Emphasis on Berries and Leaves, and Insights Gained from a Cross-Species Comparison.International journal of molecular sciences · 2021Review
- Transformation of Salicylic Acid and Its Distribution in Tea Plants (Plants (Basel, Switzerland) · 2021Article
- Magnesium Signaling in Plants.International journal of molecular sciences · 2021Review
- Nonaqueous fractionation and overexpression of fluorescent-tagged enzymes reveals the subcellular sites of L-theanine biosynthesis in tea.Plant biotechnology journal · 2021Article
- Characterization of Local Products for Their Industrial Use: The Case of Italian Potato Cultivars Analyzed by Untargeted and Targeted Methodologies.Foods (Basel, Switzerland) · 2020Article
- Metabolism of Gallic Acid and Its Distributions in Tea (International journal of molecular sciences · 2020Article
- Article
- Overexpression of Melon Tonoplast Sugar Transporter CmTST1 Improved Root Growth under High Sugar Content.International journal of molecular sciences · 2020Article
- NTRC Plays a Crucial Role in Starch Metabolism, Redox Balance, and Tomato Fruit Growth.Plant physiology · 2019Article
- Resolving subcellular plant metabolism.The Plant journal : for cell and molecular biology · 2019Article
- Non-aqueous Fractionation (NAF) for Metabolite Analysis in Subcellular Compartments ofBio-protocol · 2019Article
- Niche-specific metabolic adaptation in biotrophic and necrotrophic oomycetes is manifested in differential use of nutrients, variation in gene content, and enzyme evolution.PLoS pathogens · 2019Article
- Non-aqueous fractionation revealed changing subcellular metabolite distribution during apple fruit development.Horticulture research · 2019Article
- Cytosolic malate and oxaloacetate activate S-type anion channels in Arabidopsis guard cells.The New phytologist · 2018Article
36 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The compartmentation of metabolism in heterotrophic plant tissues is poorly understood due to the lack of data on metabolite distributions and fluxes between subcellular organelles. The main reason for this is the lack of suitable experimental methods with which intracellular metabolism can be measured. Here, we describe a nonaqueous fractionation method that allows the subcellular distributions of metabolites in developing potato (Solanum tuberosum L. cv Desiree) tubers to be calculated. In addition, we have coupled this fractionation method to a recently described gas chromatography-mass spectrometry procedure that allows the measurement of a wide range of small metabolites. To calculate the subcellular metabolite concentrations, we have analyzed organelle volumes in growing potato tubers using electron microscopy. The relative volume distributions in tubers are very similar to the ones for source leaves. More than 60% of most sugars, sugar alcohols, organic acids, and amino acids were found in the vacuole, although the concentrations of these metabolites is often higher in the cytosol. Significant amounts of the substrates for starch biosynthesis, hexose phosphates, and ATP were found in the plastid. However, pyrophosphate was located almost exclusively in the cytosol. Calculation of the mass action ratios of sucrose synthase, UDP-glucose pyrophosphorylase, phosphoglucosisomerase, and phosphoglucomutase indicate that these enzymes are close to equilibrium in developing potato tubers. However, due to the low plastidic pyrophosphate concentration, the reaction catalyzed by ADP-glucose pyrophosphorylase was estimated to be far removed from equilibrium.
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