ArticleMolecular biology of the cell2001
Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 117 citations in OpenAlex.
- Vimentin Intermediate Filaments: A Paradigm Shift From Static Structure to Dynamic Cytoplasmic Network.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- KIF5A regulates axonal repair and time-dependent axonal transport of SFPQ granules and mitochondria in human motor neurons.bioRxiv : the preprint server for biology · 2024Article
- Regulation of neurofilament length and transport by a dynamic cycle of phospho-dependent polymer severing and annealing.Molecular biology of the cell · 2023Article
- Article
- A possible mechanism for neurofilament slowing down in myelinated axon: Phosphorylation-induced variation of NF kinetics.PloS one · 2021Article
- A mechanism for neurofilament transport acceleration through nodes of Ranvier.Molecular biology of the cell · 2020Article
- Local Acceleration of Neurofilament Transport at Nodes of Ranvier.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Axonal neurofilaments exhibit frequent and complex folding behaviors.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Assembly and turnover of neurofilaments in growing axonal neurites.Biology open · 2018Article
- Kymograph analysis with high temporal resolution reveals new features of neurofilament transport kinetics.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Intermediate filament dynamics: What we can see now and why it matters.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Live-cell imaging of neurofilament transport in cultured neurons.Methods in cell biology · 2016Article
- Bidirectional actin transport is influenced by microtubule and actin stability.Cellular and molecular life sciences : CMLS · 2015Article
- Article
- Axonal transport cargo motor count versus average transport velocity: is fast versus slow transport really single versus multiple motor transport?Journal of theoretical biology · 2015Article
- Local regulation of neurofilament transport by myelinating cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Severing and end-to-end annealing of neurofilaments in neurons.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A critical reevaluation of the stationary axonal cytoskeleton hypothesis.Cytoskeleton (Hoboken, N.J.) · 2013Review
- FluoroMyelin™ Red is a bright, photostable and non-toxic fluorescent stain for live imaging of myelin.Journal of neuroscience methods · 2012Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Observations on naturally occurring gaps in the axonal neurofilament array of cultured neurons have demonstrated that neurofilament polymers move along axons in a rapid, intermittent, and highly asynchronous manner. In contrast, studies on axonal neurofilaments using laser photobleaching have not detected movement. Here, we describe a modified photobleaching strategy that does permit the direct observation of neurofilament movement. Axons of cultured neurons expressing GFP-tagged neurofilament protein were bleached by excitation with the mercury arc lamp of a conventional epifluorescence microscope for 12-60 s. The length of the bleached region ranged from 10 to 60 microm. By bleaching thin axons, which have relatively few neurofilaments, we were able to reduce the fluorescent intensity enough to allow the detection of neurofilaments that moved in from the surrounding unbleached regions. Time-lapse imaging at short intervals revealed rapid, intermittent, and highly asynchronous movement of fluorescent filaments through the bleached regions at peak rates of up to 2.8 microm/s. The kinetics of movement were very similar to our previous observations on neurofilaments moving through naturally occurring gaps, which indicates that the movement was not impaired by the photobleaching process. These results demonstrate that fluorescence photobleaching can be used to study the slow axonal transport of cytoskeletal polymers, but only if the experimental strategy is designed to ensure that rapid asynchronous movements can be detected. This may explain the failure of previous photobleaching studies to reveal the movement of neurofilament proteins and other cytoskeletal proteins in axons.
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