ArticleMolecular biology of the cell2001
Ggamma in dictyostelium: its role in localization of gbetagamma to the membrane is required for chemotaxis in shallow gradients.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Related Dictyostelium G protein subunits Gα1 and Gα2 have different roles in chemotaxis and transcription factor regulation.BMC molecular and cell biology · 2025Article
- The cAMP-induced G protein subunits dissociation monitored in live Dictyostelium cells by BRET reveals two activation rates, a positive effect of caffeine and potential role of microtubules.Cellular signalling · 2018Article
- G-Protein Dependent Signal Transduction and Ubiquitination in Dictyostelium.International journal of molecular sciences · 2017Review
- Article
- A Model for Direction Sensing in Dictyostelium discoideum: Ras Activity and Symmetry Breaking Driven by a Gβγ-Mediated, Gα2-Ric8 -- Dependent Signal Transduction Network.PLoS computational biology · 2016Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- The Dictyostelium discoideum RACK1 orthologue has roles in growth and development.Cell communication and signaling : CCS · 2014Article
- PIP₃-dependent macropinocytosis is incompatible with chemotaxis.The Journal of cell biology · 2014Article
- Diversity of heterotrimeric G-protein γ subunits in plants.BMC research notes · 2012Article
- A Gβγ effector, ElmoE, transduces GPCR signaling to the actin network during chemotaxis.Developmental cell · 2012Article
- Direct biochemical measurements of signal relay during Dictyostelium development.The Journal of biological chemistry · 2011Article
- Cellular responses to extracellular guidance cues.The EMBO journal · 2010Review
- MAP kinases have different functions in Dictyostelium G protein-mediated signaling.Cellular signalling · 2010Article
- Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity.Annual review of biophysics · 2010Review
- The Galpha4 G protein subunit interacts with the MAP kinase ERK2 using a D-motif that regulates developmental morphogenesis in Dictyostelium.Developmental biology · 2009Article
- Dynamic localization of G proteins in Dictyostelium discoideum.Journal of cell science · 2009Article
- Oscillatory signaling and network responses during the development of Dictyostelium discoideum.Ageing research reviews · 2008Review
- Developmental morphology and chemotactic responses are dependent on G alpha subunit specificity in Dictyostelium.Developmental biology · 2007Article
- Isoprenylcysteine carboxy methylation is essential for development in Dictyostelium discoideum.Molecular biology of the cell · 2007Article
- Directional sensing in eukaryotic chemotaxis: a balanced inactivation model.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
G-protein-mediated signal transduction pathways play an essential role in the developmental program of the simple eukaryotic organism Dictyostelium discoideum. Database searches have yielded 11 Galpha-subunits, a single Gbeta-subunit, but no Ggamma-subunits. We report here the purification, cDNA isolation, and functional analysis of a Ggamma-subunit. Like Gbeta, the Ggamma appears to be unique and hybridization studies show that Ggamma and Gbeta are expressed in parallel during development. Species-wide sequence comparisons of Ggamma-subunits and gamma-like domains of RGS proteins reveal short stretches of highly conserved residues as well as the common CXXL motif at the COOH-terminal of Ggammas that target Gbetagammas to plasma membrane. Overexpression of a CSVL-deleted Ggamma (GgammaDelta) in wild-type cells shifts Gbetagamma to the cytosol and selectively impairs certain G-protein-mediated signal transduction pathways. These cells are able to respond to increments in the stimulus, but are unable to sense chemoattractant gradients. They neither move directionally nor recruit PH-domains to their leading edge. Thus, a full complement of membrane-tethered Gbetagamma is required for sensing shallow gradients, but is not essential for responses to increments in extracellular stimuli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.