ArticleMolecular biology of the cell2001
Serial analysis of gene expression in Plasmodium falciparum reveals the global expression profile of erythrocytic stages and the presence of anti-sense transcripts in the malarial parasite.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed.
- New insights on Plasmodium gene expression from direct RNA sequencing.Trends in parasitology · 2026Review
- Integrative transcriptomic and machine learning approaches to decipher mitochondrial gene regulation in severe Plasmodium vivax malaria.Malaria journal · 2025Article
- Population genomics and transcriptomics of Plasmodium falciparum in Cambodia and Vietnam uncover key components of the artemisinin resistance genetic background.Nature communications · 2024Article
- Novel insights into the role of long non-coding RNA in the human malaria parasite, Plasmodium falciparum.Nature communications · 2023Article
- The role of long noncoding RNAs in malaria parasites.Trends in parasitology · 2023Review
- Emerging biology of noncoding RNAs in malaria parasites.PLoS pathogens · 2022Review
- Non-coding RNAs in malaria infection.Wiley interdisciplinary reviews. RNA · 2022Review
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- The Role of Hsp70s in the Development and Pathogenicity of Plasmodium falciparum.Advances in experimental medicine and biology · 2021Article
- The transcriptome of the rumen ciliate Entodinium caudatum reveals some of its metabolic features.BMC genomics · 2019Article
- Long Non-Coding RNAs in the Regulation of Gene Expression: Physiology and Disease.Non-coding RNA · 2019Review
- tRNA epitranscriptomics and biased codon are linked to proteome expression inMolecular systems biology · 2018Article
- Identification of a novel merozoite surface antigen of Plasmodium vivax, PvMSA180.Malaria journal · 2017Article
- Antisense long noncoding RNAs regulate var gene activation in the malaria parasite Plasmodium falciparum.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Noncoding RNAs as emerging regulators of Plasmodium falciparum virulence gene expression.Current opinion in microbiology · 2014Review
- Strand-specific RNA-Seq reveals widespread and developmentally regulated transcription of natural antisense transcripts in Plasmodium falciparum.BMC genomics · 2014Article
- The case for a rational genome-based vaccine against malaria.Frontiers in microbiology · 2014Review
- Article
- Quantitative proteomics reveals new insights into erythrocyte invasion by Plasmodium falciparum.Molecular & cellular proteomics : MCP · 2012Article
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5 authors.
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Abstract
Serial analysis of gene expression (SAGE) was applied to the malarial parasite Plasmodium falciparum to characterize the comprehensive transcriptional profile of erythrocytic stages. A SAGE library of approximately 8335 tags representing 4866 different genes was generated from 3D7 strain parasites. Basic local alignment search tool analysis of high abundance SAGE tags revealed that a majority (88%) corresponded to 3D7 sequence, and despite the low complexity of the genome, 70% of these highly abundant tags matched unique loci. Characterization of these suggested the major metabolic pathways that are used by the organism under normal culture conditions. Furthermore several tags expressed at high abundance (30% of tags matching to unique loci of the 3D7 genome) were derived from previously uncharacterized open reading frames, demonstrating the use of SAGE in genome annotation. The open platform "profiling" nature of SAGE also lead to the important discovery of a novel transcriptional phenomenon in the malarial pathogen: a significant number of highly abundant tags that were derived from annotated genes (17%) corresponded to antisense transcripts. These SAGE data were validated by two independent means, strand specific reverse transcription-polymerase chain reaction and Northern analysis, where antisense messages were detected in both asexual and sexual stages. This finding has implications for transcriptional regulation of Plasmodium gene expression.
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