ArticleMolecular biology of the cell2001
Zyxin is not colocalized with vasodilator-stimulated phosphoprotein (VASP) at lamellipodial tips and exhibits different dynamics to vinculin, paxillin, and VASP in focal adhesions.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 111 citations in OpenAlex.
- Daam-, FMNL-, and mDia-family formins jointly regulate cortical actin dynamics in melanoma cells.Cell communication and signaling : CCS · 2026Article
- Reconstituted nascent adhesion condensates drive actin polymerization on supported lipid bilayers.Science advances · 2026Article
- Decisive role of mDia-family formins in cell cortex function of highly adherent cells.Science advances · 2024Article
- Zyxin is important for the stability and function of podocytes, especially during mechanical stretch.Communications biology · 2024Article
- Profiling the responsiveness of focal adhesions of human cardiomyocytes to extracellular dynamic nano-topography.Bioactive materials · 2022Article
- A Layered View on Focal Adhesions.Biology · 2021Review
- Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion.eLife · 2020Article
- Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation.Journal of cell science · 2020Article
- Dynamics and distribution of paxillin, vinculin, zyxin and VASP depend on focal adhesion location and orientation.Scientific reports · 2019Article
- Imaging the Molecular Machines That Power Cell Migration.Methods in molecular biology (Clifton, N.J.) · 2018Article
- BMP9 Crosstalk with the Hippo Pathway Regulates Endothelial Cell Matricellular and Chemokine Responses.PloS one · 2015Article
- Rac function is crucial for cell migration but is not required for spreading and focal adhesion formation.Journal of cell science · 2013Article
- Intermediate filament-associated cytolinker plectin 1c destabilizes microtubules in keratinocytes.Molecular biology of the cell · 2013Article
- Differential effect of actomyosin relaxation on the dynamic properties of focal adhesion proteins.PloS one · 2013Article
- Article
- Nanotopography as modulator of human mesenchymal stem cell function.Biomaterials · 2012Article
- Zyxin is involved in regulation of mechanotransduction in arteriole smooth muscle cells.Frontiers in physiology · 2012Article
- Oligonucleotide microarray and QRT-PCR study of adhesion protein gene expression in acute coronary syndrome patients.Inflammation · 2010Article
- Zyxin-mediated actin assembly is required for efficient wound closure.The Journal of biological chemistry · 2010Article
- Role of VASP phosphorylation for the regulation of microglia chemotaxis via the regulation of focal adhesion formation/maturation.Molecular and cellular neurosciences · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin polymerization is accompanied by the formation of protein complexes that link extracellular signals to sites of actin assembly such as membrane ruffles and focal adhesions. One candidate recently implicated in these processes is the LIM domain protein zyxin, which can bind both Ena/vasodilator-stimulated phosphoprotein (VASP) proteins and the actin filament cross-linking protein alpha-actinin. To characterize the localization and dynamics of zyxin in detail, we generated both monoclonal antibodies and a green fluorescent protein (GFP)-fusion construct. The antibodies colocalized with ectopically expressed GFP-VASP at focal adhesions and along stress fibers, but failed to label lamellipodial and filopodial tips, which also recruit Ena/VASP proteins. Likewise, neither microinjected, fluorescently labeled zyxin antibodies nor ectopically expressed GFP-zyxin were recruited to these latter sites in live cells, whereas both probes incorporated into focal adhesions and stress fibers. Comparing the dynamics of zyxin with that of the focal adhesion protein vinculin revealed that both proteins incorporated simultaneously into newly formed adhesions. However, during spontaneous or induced focal adhesion disassembly, zyxin delocalization preceded that of either vinculin or paxillin. Together, these data identify zyxin as an early target for signals leading to adhesion disassembly, but exclude its role in recruiting Ena/VASP proteins to the tips of lamellipodia and filopodia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.