ArticleThe EMBO journal2001
Antagonistic effects of T-Ag and VP16 reveal a role for RNA pol II elongation on alternative splicing.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 143 citations in OpenAlex.
- Circular RNAs increase during vascular cell differentiation and are biomarkers for vascular disease.Cardiovascular research · 2025Article
- Alternative Splicing in Embryo Implantation.Maternal-fetal medicine (Wolters Kluwer Health, Inc.) · 2025Review
- Age-dependent regulation of ELP1 exon 20 splicing in Familial Dysautonomia by RNA Polymerase II kinetics and chromatin structure.PloS one · 2024Article
- Alternative splicing diversifies the skeletal muscle transcriptome during prolonged spaceflight.Skeletal muscle · 2022Article
- Regulation of RNA Splicing: Aberrant Splicing Regulation and Therapeutic Targets in Cancer.Cells · 2021Review
- Reciprocal regulation between alternative splicing and the DNA damage response.Genetics and molecular biology · 2020Article
- A pilot study of alternative TrkAIII splicing in Merkel cell carcinoma: a potential oncogenic mechanism and novel therapeutic target.Journal of experimental & clinical cancer research : CR · 2019Article
- Past, present, and future of circRNAs.The EMBO journal · 2019Review
- Smu1 and RED are required for activation of spliceosomal B complexes assembled on short introns.Nature communications · 2019Article
- Alternative Splicing and Transcription Elongation in Plants.Frontiers in plant science · 2019Review
- Beyond Transcription: Roles of Transcription Factors in Pre-mRNA Splicing.Chemical reviews · 2018Review
- Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast.Genome research · 2018Article
- DNA-Encoded Chromatin Structural Intron Boundary Signals Identify Conserved Genes with Common Function.International journal of genomics · 2015Article
- Article
- Alternative splicing of type II procollagen: IIB or not IIB?Connective tissue research · 2014Review
- RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing.Nucleic acids research · 2014Article
- Variation in chromatin accessibility in human kidney cancer links H3K36 methyltransferase loss with widespread RNA processing defects.Genome research · 2014Article
- CRE promoter sites modulate alternative splicing via p300-mediated histone acetylation.RNA biology · 2014Article
- Tissue-specific and SRSF1-dependent splicing of fibronectin, a matrix protein that controls host cell invasion.Molecular biology of the cell · 2013Article
- Intragenic epigenetic changes modulate NCAM alternative splicing in neuronal differentiation.The EMBO journal · 2013Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we investigate the promoter control of alternative splicing by studying two transcriptional activators on templates under replicating conditions. SV40 large T-antigen (T-Ag) activates template replication only 2-fold but transcription 25-fold. T-Ag-mediated replication, reported to inhibit RNA polymerase II elongation, provokes a 10- to 30-fold increase in the inclusion of the fibronectin EDI exon into mature mRNA. The T-Ag effect is exon specific, occurs in cis and depends strictly on DNA replication and not on cell transformation. VP16, an activator of transcriptional initiation and elongation, has a similar effect on transcription but the opposite effect on splicing: EDI inclusion is inhibited by 35-fold. VP16 completely reverts the T-Ag effect, but a VP16 mutant with reduced elongation ability provokes only partial reversion. Both T-Ag and VP16 promote conspicuous co-localization of mRNA with nuclear speckles that contain the SR protein SF2/ASF, a positive regulator of EDI inclusion. Therefore, we conclude that co-localization of transcripts and speckles is not sufficient to stimulate EDI inclusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.