ArticleThe EMBO journal2001
Rho1p and Cdc42p act after Ypt7p to regulate vacuole docking.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 109 citations in OpenAlex.
- Endolysosomal trafficking controls yolk granule biogenesis in vitellogenic Drosophila oocytes.PLoS genetics · 2024Article
- A Protein-Protein Interaction Analysis Suggests a Wide Range of New Functions for the p21-Activated Kinase (PAK) Ste20.International journal of molecular sciences · 2023Article
- Stage-Specific Genetic Interaction betweenInternational journal of molecular sciences · 2022Article
- Article
- Cdc42 GTPase regulates ESCRTs in nuclear envelope sealing and ER remodeling.The Journal of cell biology · 2020Article
- Yeast translation elongation factor-1A binds vacuole-localized Rho1p to facilitate membrane integrity through F-actin remodeling.The Journal of biological chemistry · 2015Article
- Plant vacuole morphology and vacuolar trafficking.Frontiers in plant science · 2014Review
- Rho GTPases, phosphoinositides, and actin: a tripartite framework for efficient vesicular trafficking.Small GTPases · 2014Review
- The yeast ATP-binding cassette (ABC) transporter Ycf1p enhances the recruitment of the soluble SNARE Vam7p to vacuoles for efficient membrane fusion.The Journal of biological chemistry · 2013Article
- Cdk1-dependent control of membrane-trafficking dynamics.Molecular biology of the cell · 2012Article
- Cell polarization and cytokinesis in budding yeast.Genetics · 2012Review
- Regulation of vacuolar H+-ATPase activity by the Cdc42 effector Ste20 in Saccharomyces cerevisiae.Eukaryotic cell · 2012Article
- Yeast lipin 1 orthologue pah1p regulates vacuole homeostasis and membrane fusion.The Journal of biological chemistry · 2012Article
- GTPase networks in membrane traffic.Annual review of biochemistry · 2012Review
- Endosomal Na+ (K+)/H+ exchanger Nhx1/Vps44 functions independently and downstream of multivesicular body formation.The Journal of biological chemistry · 2011Article
- Endosome maturation.The EMBO journal · 2011Review
- The Rho1 GTPase acts together with a vacuolar glutathione S-conjugate transporter to protect yeast cells from oxidative stress.Genetics · 2011Article
- Exo-endocytic trafficking and the septin-based diffusion barrier are required for the maintenance of Cdc42p polarization during budding yeast asymmetric growth.Molecular biology of the cell · 2011Article
- HOPS prevents the disassembly of trans-SNARE complexes by Sec17p/Sec18p during membrane fusion.The EMBO journal · 2010Article
- Natamycin inhibits vacuole fusion at the priming phase via a specific interaction with ergosterol.Antimicrobial agents and chemotherapy · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rho GTPases, which control polarized cell growth through cytoskeletal reorganization, have recently been implicated in the control of endo- and exocytosis. We now report that both Rho1p and Cdc42p have a direct role in mediating the docking stage of homotypic vacuole fusion. Vacuoles prepared from strains with temperature-sensitive alleles of either Rho1p or Cdc42p are thermolabile for fusion. RhoGDI (Rdi1p), which extracts Rho1p and Cdc42p from the vacuole membrane, blocks vacuole fusion. The Rho GTPases can not fulfill their function as long as priming and Ypt7p-dependent tethering are inhibited. However, reactions that are reversibly blocked after docking by the calcium chelator BAPTA have passed the point of sensitivity to Rdi1p. Extraction and removal of Ypt7p, Rho1p and Cdc42p from docked vacuoles (by Gdi1p, Gyp7p and Rdi1p) does not impede subsequent membrane fusion, which is still sensitive to GTPgammaS. Thus, multiple GTPases act in a defined sequence to regulate the docking steps of vacuole fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.