ArticleThe Plant cell2001
Genomics analysis of genes expressed in maize endosperm identifies novel seed proteins and clarifies patterns of zein gene expression.
Article in The Plant cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
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Who cites it
115 citing papers in PubMed, 265 citations in OpenAlex.
- Unveiling the Link Between Expression of Aspartate Kinase2 (ask2) Gene and Accumulation of Methionine in Developing Maize Kernels.Biochemical genetics · 2026Article
- ZmMYB127 controls maize endosperm filling via dual-transcriptional regulation to improve grain yield and quality.Nature plants · 2026Article
- Opaque-2 induced zein reduction and lysine increase suggest a route to quality protein sweet corn.Frontiers in plant science · 2026Article
- Genetic characterization of commercial spring maize germplasm in Northern China for hybrid breeding and trait improvement.Frontiers in plant science · 2026Article
- The phylotranscriptomic profile of angiosperm seed development follows a reverse hourglass pattern.The Plant cell · 2025Article
- Identification of QTLs associated withFrontiers in plant science · 2025Article
- Temporal profile of amino acids and protein fractions in the developing kernel of maize germplasm.Scientific reports · 2024Article
- Rapid Tryptophan Assay as a Screening Procedure for Quality Protein Maize.Molecules (Basel, Switzerland) · 2024Article
- Understanding grain development in the Poaceae family by comparing conserved and distinctive pathways through omics studies in wheat and maize.Frontiers in plant science · 2024Review
- Imaging the ER and Endomembrane System in Cereal Endosperm.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Conformations of a highly expressed Z19 α-zein studied with AlphaFold2 and MD simulations.PloS one · 2024Article
- Autophagy during maize endosperm development dampens oxidative stress and promotes mitochondrial clearance.Plant physiology · 2023Article
- Generation of multi-layered protein bodies inFrontiers in plant science · 2023Article
- Endosperm-specific accumulation of human α-lactalbumin increases seed lysine content in maize.Plant cell reports · 2022Article
- Engineered FungusJournal of fungi (Basel, Switzerland) · 2022Article
- Multiscale imaging reveals the presence of autophagic vacuoles in developing maize endosperm.Frontiers in plant science · 2022Article
- Dynamic Transcriptome-Based Weighted Gene Co-expression Network Analysis Reveals Key Modules and Hub Genes Associated With the Structure and Nutrient Formation of Endosperm for Wax Corn.Frontiers in plant science · 2022Article
- Maize Endosperm Development: Tissues, Cells, Molecular Regulation and Grain Quality Improvement.Frontiers in plant science · 2022Review
- Microautophagy Mediates Vacuolar Delivery of Storage Proteins in Maize Aleurone Cells.Frontiers in plant science · 2022Article
- Two γ-zeins induce the unfolded protein response.Plant physiology · 2021Article
55 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We analyzed cDNA libraries from developing endosperm of the B73 maize inbred line to evaluate the expression of storage protein genes. This study showed that zeins are by far the most highly expressed genes in the endosperm, but we found an inverse relationship between the number of zein genes and the relative amount of specific mRNAs. Although alpha-zeins are encoded by large multigene families, only a few of these genes are transcribed at high or detectable levels. In contrast, relatively small gene families encode the gamma- and delta-zeins, and members of these gene families, especially the gamma-zeins, are highly expressed. Knowledge of expressed storage protein genes allowed the development of DNA and antibody probes that distinguish between closely related gene family members. Using in situ hybridization, we found differences in the temporal and spatial expression of the alpha-, gamma-, and delta-zein gene families, which provides evidence that gamma-zeins are synthesized throughout the endosperm before alpha- and delta-zeins. This observation is consistent with earlier studies that suggested that gamma-zeins play an important role in prolamin protein body assembly. Analysis of endosperm cDNAs also revealed several previously unidentified proteins, including a 50-kD gamma-zein, an 18-kD alpha-globulin, and a legumin-related protein. Immunolocalization of the 50-kD gamma-zein showed this protein to be located at the surface of prolamin-containing protein bodies, similar to other gamma-zeins. The 18-kD alpha-globulin, however, is deposited in novel, vacuole-like organelles that were not described previously in maize endosperm.
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