ArticleOncogene2001
Induction of ser15 and lys382 modifications of p53 by blockage of transcription elongation.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
- Human embryonic stem cells display a pronounced sensitivity to the cyclin dependent kinase inhibitor Roscovitine.BMC molecular and cell biology · 2019Article
- DNA damage-induced ATM- and Rad-3-related (ATR) kinase activation in non-replicating cells is regulated by the XPB subunit of transcription factor IIH (TFIIH).The Journal of biological chemistry · 2017Article
- Human p53 interacts with the elongating RNAPII complex and is required for the release of actinomycin D induced transcription blockage.Scientific reports · 2017Article
- DNA damage and the balance between survival and death in cancer biology.Nature reviews. Cancer · 2016Review
- Non-Coding RNA: Sequence-Specific Guide for Chromatin Modification and DNA Damage Signaling.Frontiers in genetics · 2015Review
- Proteomic analysis of arginine methylation sites in human cells reveals dynamic regulation during transcriptional arrest.Molecular & cellular proteomics : MCP · 2014Article
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- CSA and CSB proteins interact with p53 and regulate its Mdm2-dependent ubiquitination.Cell cycle (Georgetown, Tex.) · 2011Article
- Transcriptional regulation of HIV-1 gene expression by p53.Cell cycle (Georgetown, Tex.) · 2010Article
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- Psoralen-induced DNA interstrand cross-links block transcription and induce p53 in an ataxia-telangiectasia and rad3-related-dependent manner.Molecular pharmacology · 2009Article
- Versatile functions of p53 protein in multicellular organisms.Biochemistry. Biokhimiia · 2007Review
- RPA and ATR link transcriptional stress to p53.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The p53-targeting human phosphatase hCdc14A interacts with the Cdk1/cyclin B complex and is differentially expressed in human cancers.Molecular cancer · 2006Article
- Nuclear accumulation of p53 following inhibition of transcription is not due to diminished levels of MDM2.Oncogene · 2004Article
- Role of the C-terminal domain of RNA polymerase II in U2 snRNA transcription and 3' processing.Molecular and cellular biology · 2004Article
- Mitochondrial DNA damage and its consequences for mitochondrial gene expression.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Blockage of transcription has been shown to induce the tumor suppressor p53 in human cells. We here show that RNA synthesis inhibitors blocking the phosphorylation of the carboxyl terminal domain (CTD) of RNA polymerase II, such as DRB and H7, induced rapid nuclear accumulation of p53 proteins that were not phosphorylated at ser15 or acetylated at lys382. In contrast, agents that inhibit the elongation phase of transcription, such as UV light, camptothecin or actinomycin D, induced the accumulation of nuclear p53 proteins that were modified at both of these sites. Furthermore, using a panel of DNA repair-deficient cells we show that persistent DNA lesions in the transcribed strand of active genes are responsible for the induction of the ser15 and lys382 modifications following UV-irradiation. We conclude that inhibition of transcription is sufficient for the accumulation of p53 in the nucleus regardless of whether the ser15 site of p53 is phosphorylated or not. Importantly, blockage of the elongation phase of transcription triggers a distinct signaling pathway leading to p53 modifications on ser15 and lys382. We propose that the elongating RNA polymerase complex may act as a sensor of DNA damage and as an integrator of cellular stress signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.