ArticleOncogene2001
Common and reversible regulation of wild-type p53 function and of ribosomal biogenesis by protein kinases in human cells.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 60 citations in OpenAlex.
- SLFN11-mediated ribosome biogenesis impairment induces TP53-independent apoptosis.Molecular cell · 2025Article
- CX-5461 causes nucleolar compaction, alteration of peri- and intranucleolar chromatin arrangement, an increase in both heterochromatin and DNA damage response.Scientific reports · 2022Article
- Review
- Inhibition of p53 inhibitors: progress, challenges and perspectives.Journal of molecular cell biology · 2019Review
- Nucleolar Stress: hallmarks, sensing mechanism and diseases.Cell stress · 2018Review
- Alu element-containing RNAs maintain nucleolar structure and function.The EMBO journal · 2015Article
- New insights into nucleolar structure and function.F1000prime reports · 2015Review
- Strategic cell-cycle regulatory features that provide mammalian cells with tunable G1 length and reversible G1 arrest.PloS one · 2012Article
- Controlling subcellular delivery to optimize therapeutic effect.Therapeutic delivery · 2010Review
- Roscovitine synergizes with conventional chemo-therapeutic drugs to induce efficient apoptosis of human colorectal cancer cells.World journal of gastroenterology · 2008Article
- Nucleolar targeting of hepatitis delta antigen abolishes its ability to initiate viral antigenomic RNA replication.Journal of virology · 2008Article
- Nucleolus: the fascinating nuclear body.Histochemistry and cell biology · 2008Review
- Versatile functions of p53 protein in multicellular organisms.Biochemistry. Biokhimiia · 2007Review
- The nucleolus: a model for the organization of nuclear functions.Histochemistry and cell biology · 2006Review
- Compartmentation of the nucleolar processing proteins in the granular component is a CK2-driven process.Molecular biology of the cell · 2006Article
- Nucleolus: from structure to dynamics.Histochemistry and cell biology · 2006Review
- The small subunit processome is required for cell cycle progression at G1.Molecular biology of the cell · 2004Article
- Nuclear accumulation of p53 following inhibition of transcription is not due to diminished levels of MDM2.Oncogene · 2004Article
- Disruption of the nucleolus mediates stabilization of p53 in response to DNA damage and other stresses.The EMBO journal · 2003Article
- Human p14(Arf): an exquisite sensor of morphological changes and of short-lived perturbations in cell cycle and in nucleolar function.Oncogene · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two specific inhibitors of cyclin-dependent kinase 2 (Cdk2), roscovitine and olomoucine, have been shown recently to induce nuclear accumulation of wt p53 and nucleolar unravelling in interphase human untransformed IMR-90 and breast tumor-derived MCF-7 cells. Here, we show that the early response of MCF-7 cells to roscovitine is fully reversible since a rapid restoration of nucleolar organization followed by an induction of p21(WAF1/CIP1), a downregulation of nuclear wt p53 and normal cell cycle resumption occurs if the compound is removed after 4 h. Interestingly, similar reversible effects are also induced by the casein kinase II (CKII) inhibitor, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. Upon short-term treatment also, both compounds significantly, but reversibly, reduce the level of 45S precursor ribosomal RNA. Cells exposed to the two types of protein kinase inhibitors for longer times keep exhibiting altered nucleolar and wt p53 features, yet they strikingly differentiate in that most roscovitine-treated cells fail to ever accumulate high levels of p21(WAF1/CIP1) in contrast with DRB-treated ones. In both cases, however, the cells eventually fall into an irreversible state and die. Moreover, we found that constitutive overexpression of p21(WAF1/CIP1) alters the nucleolar unravelling process in the presence of DRB, but not of roscovitine, suggesting a role for this physiological Cdk inhibitor in the regulation of nucleolar function. Our data also support the notion that both roscovitine- and DRB-sensitive protein kinases, probably including Cdk2 and CKII, via their dual implication in the p53-Rb pathway and in ribosomal biogenesis, would participate in coupling cell growth with cell division.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.