ArticleGene therapy2001
Adenoviral vector-mediated insulin gene transfer in the mouse pancreas corrects streptozotocin-induced hyperglycemia.
Article in Gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- Mapping and targeted viral activation of pancreatic nerves in mice reveal their roles in the regulation of glucose metabolism.Nature biomedical engineering · 2022Article
- Focus on the Lymphatic Route to Optimize Drug Delivery in Cardiovascular Medicine.Pharmaceutics · 2021Review
- In VivoInternational journal of molecular sciences · 2019Article
- Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation.eLife · 2015Article
- Nondividing, postpubertal rat sertoli cells resumed proliferation after transplantation.Biology of reproduction · 2014Article
- Site-targeted non-viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice.Biotechnology journal · 2013Article
- Ligand-bound thyroid hormone receptor contributes to reprogramming of pancreatic acinar cells into insulin-producing cells.The Journal of biological chemistry · 2013Article
- Radio-wave heating of iron oxide nanoparticles can regulate plasma glucose in mice.Science (New York, N.Y.) · 2012Article
- Intra-arterial targeted islet-specific expression of Sirt1 protects β cells from streptozotocin-induced apoptosis in mice.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Liganded thyroid hormone receptor-alpha enhances proliferation of pancreatic beta-cells.The Journal of biological chemistry · 2010Article
- Delivery of a therapeutic protein by immune-privileged Sertoli cells.Cell transplantation · 2010Article
- Gene therapy for type 1 diabetes: is it ready for the clinic?Immunologic research · 2006Review
- Molecular targeting of pancreatic disorders.World journal of surgery · 2005Review
- Current status and prospects for gene and cell therapeutics for type 1 diabetes mellitus.Reviews in endocrine & metabolic disorders · 2003Review
- Gene- and cell-based therapeutics for type I diabetes mellitus.Gene therapy · 2003Review
- Human insulin production and amelioration of diabetes in mice by electrotransfer-enhanced plasmid DNA gene transfer to the skeletal muscle.Gene therapy · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Therapy for type 1 diabetes consists of tight blood glucose (BG) control to minimize complications. Current treatment relies on multiple insulin injections or an insulin pump placement, beta-cell or whole pancreas transplantation. All approaches have significant limitations and have led to the realization that novel treatment strategies are needed. Pancreatic acinar cells have features that make them a good target for insulin gene transfer. They are not subject to autoimmune attack, a problem with pancreas or islets transplantation, they are avidly transduced by recombinant adenoviral vectors, and capable of exporting a variety of peptides into the portal circulation. Recombinant adenoviral vectors were engineered to express either wild-type or furin-modified human insulin cDNA (AdCMVhInsM). Immunodeficient mice were made diabetic with streptozotocin and injected intrapancreatically with the vectors. BG and blood insulin levels have normalized after administration of AdCMVhInsM. Immunohistochemistry and electron microscopy showed the presence of insulin in acinar cells throughout the pancreas and localization of insulin molecules to acinar cell vesicles. The data clearly establish a relationship between intrapancreatic vector administration, decreased BG and elevated blood insulin levels. The findings support the use of pancreatic acinar cells to express and secrete insulin into the blood stream.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.