ArticleGene therapy2001
Prediction and optimization of gene transfection and drug delivery by electroporation.
Article in Gene therapy, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed.
- Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection.Biotechnology reports (Amsterdam, Netherlands) · 2023Article
- Genetic Modification of Mesenchymal Stem Cells for Neurological Disease Therapy: What Effects Does it Have on Phenotype/Cell Behavior, Determining Their Effectiveness?Molecular diagnosis & therapy · 2020Review
- Mesenchymal Stem Cells as Therapeutic Agents and Novel Carriers for the Delivery of Candidate Genes in Acute Kidney Injury.Stem cells international · 2020Review
- Enhancements of Cancer Cell Damage Efficiencies in Photothermal and Photodynamic Processes through Cell Perforation and Preheating with Surface Plasmon Resonance of Gold Nanoring.Molecules (Basel, Switzerland) · 2018Article
- Exosomes and their Application in Biomedical Field: Difficulties and Advantages.Molecular neurobiology · 2018Review
- Repeated photoporation with graphene quantum dots enables homogeneous labeling of live cells with extrinsic markers for fluorescence microscopy.Light, science & applications · 2018Article
- Synergistic combinations of short high-voltage pulses and long low-voltage pulses enhance irreversible electroporation efficacy.Scientific reports · 2017Article
- Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound.Scientific reports · 2016Article
- Molecular dynamic simulation of transmembrane pore growth.The Journal of membrane biology · 2013Article
- Intracellular protein delivery and gene transfection by electroporation using a microneedle electrode array.Small (Weinheim an der Bergstrasse, Germany) · 2012Article
- Site-specific sonoporation of human melanoma cells at the cellular level using high lateral-resolution ultrasonic micro-transducer arrays.Biosensors & bioelectronics · 2011Article
- Control of the release of freely diffusing molecules in single-cell electroporation.Analytical chemistry · 2009Article
- Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode.Gene therapy · 2007Article
- Hepatocyte-targeted gene transfer by combination of vascularly delivered plasmid DNA and in vivo electroporation.Gene therapy · 2005Article
- Tissue electroporation: quantification and analysis of heterogeneous transport in multicellular environments.Biophysical journal · 2004Article
- A model for the analysis of nonviral gene therapy.Gene therapy · 2003Article
- An experimental and theoretical analysis of ultrasound-induced permeabilization of cell membranes.Biophysical journal · 2003Article
- In vitro transfection of plasmid DNA by amine derivatives of gelatin accompanied with ultrasound irradiation.Pharmaceutical research · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although electroporation is widely used for laboratory gene transfection and gaining increased importance for nonviral gene therapy, it is generally employed using trial-and-error optimization schemes for lack of methods to predict electroporation's effects on cells. Therefore, we used a statistical approach to quantitatively predict molecular uptake and cell viability following electroporation and show that it predicts both in vitro and in vivo results for a wide range of molecules, including DNA, in 60 different cell types. Mechanistically, this broad predictive ability suggests that electroporation is mediated primarily by lipid bilayer structure and only secondarily by cell-specific characteristics. For gene therapy applications, this approach should facilitate rational design of electroporation protocols.
Indexed as
Identifiers
11593359What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.