ArticleProceedings of the National Academy of Sciences of the United States of America2001
Nuclear relocation of a transactivator subunit precedes target gene activation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 136 citations in OpenAlex.
- MAFF Suppresses Necroptosis in Pancreatic Cancer via the GATA4-MLKL Axis.Cancer science · 2026Article
- Igh nuclear speckle association promotes V(D)J recombination.Nature communications · 2026Article
- Nuclear position modulates long-range chromatin interactions.PLoS genetics · 2022Article
- Review
- HTLV-1 basic leucine zipper factor protects cells from oxidative stress by upregulating expression of Heme Oxygenase I.PLoS pathogens · 2019Article
- Heterochromatin and the molecular mechanisms of 'parent-of-origin' effects in animals.Journal of biosciences · 2016Review
- Regulation and function of the NFE2 transcription factor in hematopoietic and non-hematopoietic cells.Cellular and molecular life sciences : CMLS · 2015Review
- Olfactory receptor genes expressed in distinct lineages are sequestered in different nuclear compartments.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- β-Globin cis-elements determine differential nuclear targeting through epigenetic modifications.The Journal of cell biology · 2013Article
- Genomic cis-acting Sequences Improve Expression and Establishment of a Nonviral Vector.Molecular therapy. Nucleic acids · 2013Article
- Chromatin loop formation in the β-globin locus and its role in globin gene transcription.Molecules and cells · 2012Review
- Association of the interferon-β gene with pericentromeric heterochromatin is dynamically regulated during virus infection through a YY1-dependent mechanism.Nucleic acids research · 2012Article
- Centromere architecture breakdown induced by the viral E3 ubiquitin ligase ICP0 protein of herpes simplex virus type 1.PloS one · 2012Article
- HSV-1 genome subnuclear positioning and associations with host-cell PML-NBs and centromeres regulate LAT locus transcription during latency in neurons.PLoS pathogens · 2012Article
- Specific positioning of the casein gene cluster in active nuclear domains in luminal mammary epithelial cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Molecular determinants for small Maf protein control of platelet production.Molecular and cellular biology · 2011Article
- Multiple functions of Ldb1 required for beta-globin activation during erythroid differentiation.Blood · 2010Article
- Article
- Nuclear stress bodies.Cold Spring Harbor perspectives in biology · 2010Review
- Epigenetic modifications in 3D: nuclear organization of the differentiating mammary epithelial cell.Journal of mammary gland biology and neoplasia · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Murine erythroleukemia (MEL) cells are a model system to study reorganization of the eukaryotic nucleus during terminal differentiation. Upon chemical induction, MEL cells undergo erythroid differentiation, leading to activation of globin gene expression. Both processes strongly depend on the transcriptional activator NF-E2. Before induction of differentiation, both subunits of the NF-E2 heterodimer are present, but little DNA-binding activity is detectable. Using immunofluorescence microscopy, we show that the two NF-E2 subunits occupy distinct nuclear compartments in uninduced MEL cells; the smaller subunit NF-E2p18 is found primarily in the centromeric heterochromatin compartment, whereas the larger subunit NF-E2p45 occupies the euchromatin compartment. Concomitant with the commitment period of differentiation that precedes globin gene activation, NF-E2p18, along with other transcriptional repressors, relocates to the euchromatin compartment. Thus, relocation of NF-E2 p18 may be a rate-limiting step in formation of an active NF-E2 complex. To understand the mechanisms of NF-E2 localization, we show that centromeric targeting of NF-E2p18 requires dimerization, but not with an erythroid-specific partner, and that the transactivation domain of NF-E2p45 may be necessary and sufficient to prevent its localization in centromeric heterochromatin. Finally, using fluorescence in situ hybridization, we show that, upon differentiation, the beta-globin gene loci relocate away from heterochromatin compartments to euchromatin. This relocation correlates with both transcriptional activation of the globin locus and relocation of NF-E2p18 away from heterochromatin, suggesting that these processes are linked.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.