ArticleThe Journal of cell biology2001
Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed, 112 citations in OpenAlex.
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- Orchestration of SARS-CoV-2 Nsp4 and host cell ESCRT proteins induces morphological changes of the endoplasmic reticulum.Molecular biology of the cell · 2025Article
- A feedforward loop between JAK/STAT downstream target p115 and STAT in germline stem cells.Stem cell reports · 2023Article
- The factory, the antenna and the scaffold: the three-way interplay between the Golgi, cilium and extracellular matrix underlying tissue function.Biology open · 2023Review
- Golgi Dysfunctions in Ciliopathies.Cells · 2022Review
- Hypothalamic orexinergic neuron changes during the hibernation of the Syrian hamster.Frontiers in neuroanatomy · 2022Article
- Mapping the molecular basis for growth related phenotypes in industrial producer CHO cell lines using differential proteomic analysis.BMC biotechnology · 2021Article
- Giantin is required for intracellular N-terminal processing of type I procollagen.The Journal of cell biology · 2021Article
- The Role of GM130 in Nervous System Diseases.Frontiers in neurology · 2021Review
- A Novel 65-bp Indel in theAnimals : an open access journal from MDPI · 2020Article
- Golgi organization is regulated by proteasomal degradation.Nature communications · 2020Article
- Article
- The Physiological Functions of the Golgin Vesicle Tethering Proteins.Frontiers in cell and developmental biology · 2019Review
- Probing the contribution of individual polypeptide GalNAc-transferase isoforms to theThe Journal of biological chemistry · 2018Article
- PI3K class II α regulates δ-opioid receptor export from theMolecular biology of the cell · 2017Article
- Golgb1 regulates protein glycosylation and is crucial for mammalian palate development.Development (Cambridge, England) · 2016Article
- Induced oligomerization targets Golgi proteins for degradation in lysosomes.Molecular biology of the cell · 2015Article
- Structural basis for the interaction between the Golgi reassembly-stacking protein GRASP65 and the Golgi matrix protein GM130.The Journal of biological chemistry · 2015Article
- The golgin GMAP-210 is required for efficient membrane trafficking in the early secretory pathway.Journal of cell science · 2015Article
- p115-SNARE interactions: a dynamic cycle of p115 binding monomeric SNARE motifs and releasing assembled bundles.Traffic (Copenhagen, Denmark) · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Inhibition of the putative coatomer protein I (COPI) vesicle tethering complex, giantin-p115-GM130, may contribute to mitotic Golgi breakdown. However, neither this, nor the role of the giantin-p115-GM130 complex in the maintenance of Golgi structure has been demonstrated in vivo. Therefore, we generated antibodies directed against the mapped binding sites in each protein of the complex and injected these into mammalian tissue culture cells. Surprisingly, the injected anti-p115 and antigiantin antibodies caused proteasome-mediated degradation of the corresponding antigens. Reduction of p115 levels below detection led to COPI-dependent Golgi fragmentation and apparent accumulation of Golgi-derived vesicles. In contrast, neither reduction of giantin below detectable levels, nor inhibition of p115 binding to GM130, had any detectable effect on Golgi structure or Golgi reassembly after cell division or brefeldin A washout. These observations indicate that inhibition of p115 can induce a mitotic-like Golgi disassembly, but its essential role in Golgi structure is independent of its Golgi-localized binding partners giantin and GM130.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.