ArticleJournal of bacteriology2001
Bacterial two-hybrid analysis of interactions between region 4 of the sigma(70) subunit of RNA polymerase and the transcriptional regulators Rsd from Escherichia coli and AlgQ from Pseudomonas aeruginosa.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 60 citations in OpenAlex.
- Optimized Quantitative Bacterial Two-Hybrid (qB2H) for Protein-Protein Interaction Assessment.Computational and structural biotechnology journal · 2026Article
- The opposite effects of stringent response on phage infection ofmicroLife · 2026Article
- Optimization of a bacterial three-hybrid assay through in vivo titration of an RNA-DNA adapter protein.RNA (New York, N.Y.) · 2021Article
- NovelViruses · 2020Article
- Coordinated Regulation of Rsd and RMF for Simultaneous Hibernation of Transcription Apparatus and Translation Machinery in Stationary-PhaseFrontiers in genetics · 2019Review
- Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library.BMC genomics · 2017Article
- Bacterial Sigma Factors and Anti-Sigma Factors: Structure, Function and Distribution.Biomolecules · 2015Review
- Genes required for and effects of alginate overproduction induced by growth of Pseudomonas aeruginosa on Pseudomonas isolation agar supplemented with ammonium metavanadate.Journal of bacteriology · 2013Article
- Anti-Sigma Factors in E. coli: Common Regulatory Mechanisms Controlling Sigma Factors Availability.Current genomics · 2013Article
- Evidence for sigma factor competition in the regulation of alginate production by Pseudomonas aeruginosa.PloS one · 2013Article
- Transcriptional control in the prereplicative phase of T4 development.Virology journal · 2010Review
- Structure and protein-protein interaction studies on Chlamydia trachomatis protein CT670 (YscO Homolog).Journal of bacteriology · 2010Article
- A two-subunit bacterial sigma-factor activates transcription in Bacillus subtilis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Direct activator/co-activator interaction is essential for bacteriophage T4 middle gene expression.Molecular microbiology · 2009Article
- A regulator from Chlamydia trachomatis modulates the activity of RNA polymerase through direct interaction with the beta subunit and the primary sigma subunit.Genes & development · 2009Article
- The bacteriophage T4 AsiA protein contacts the beta-flap domain of RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Mutagenesis of region 4 of sigma 28 from Chlamydia trachomatis defines determinants for protein-protein and protein-DNA interactions.Journal of bacteriology · 2009Article
- Rsd family proteins make simultaneous interactions with regions 2 and 4 of the primary sigma factor.Molecular microbiology · 2008Article
- Differential mechanisms of binding of anti-sigma factors Escherichia coli Rsd and bacteriophage T4 AsiA to E. coli RNA polymerase lead to diverse physiological consequences.Journal of bacteriology · 2008Article
- Crystal structure of the Escherichia coli regulator of sigma70, Rsd, in complex with sigma70 domain 4.Journal of molecular biology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A number of transcriptional regulators mediate their effects through direct contact with the sigma(70) subunit of Escherichia coli RNA polymerase (RNAP). In particular, several regulators have been shown to contact a C-terminal portion of sigma(70) that harbors conserved region 4. This region of sigma contains a putative helix-turn-helix DNA-binding motif that contacts the -35 element of sigma(70)-dependent promoters directly. Here we report the use of a recently developed bacterial two-hybrid system to study the interaction between the putative anti-sigma factor Rsd and the sigma(70) subunit of E. coli RNAP. Using this system, we found that Rsd can interact with an 86-amino-acid C-terminal fragment of sigma(70) and also that amino acid substitution R596H, within region 4 of sigma(70), weakens this interaction. We demonstrated the specificity of this effect by showing that substitution R596H does not weaken the interaction between sigma and two other regulators shown previously to contact region 4 of sigma(70). We also demonstrated that AlgQ, a homolog of Rsd that positively regulates virulence gene expression in Pseudomonas aeruginosa, can contact the C-terminal region of the sigma(70) subunit of RNAP from this organism. We found that amino acid substitution R600H in sigma(70) from P. aeruginosa, corresponding to the R596H substitution in E. coli sigma(70), specifically weakens the interaction between AlgQ and sigma(70). Taken together, our findings suggest that Rsd and AlgQ contact similar surfaces of RNAP present in region 4 of sigma(70) and probably regulate gene expression through this contact.
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