ArticleJournal of bacteriology2001
An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
- Molecular model of a sensor of two-component signaling system.Scientific reports · 2021Article
- Signal transduction in photoreceptor histidine kinases.Protein science : a publication of the Protein Society · 2019Review
- The Blue-Green Sensory Rhodopsin SRM from Haloarcula marismortui Attenuates Both Phototactic Responses Mediated by Sensory Rhodopsin I and II in Halobacterium salinarum.Scientific reports · 2019Article
- Signaling and Adaptation Modulate the Dynamics of the Photosensoric Complex of Natronomonas pharaonis.PLoS computational biology · 2015Article
- Fluorescence spectroscopy of rhodopsins: insights and approaches.Biochimica et biophysica acta · 2014Review
- What site-directed labeling studies tell us about the mechanism of rhodopsin activation and G-protein binding.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2010Review
- Photoreactions and structural changes of anabaena sensory rhodopsin.Sensors (Basel, Switzerland) · 2009Article
- Mutational analysis of the connector segment in the HAMP domain of Tsr, the Escherichia coli serine chemoreceptor.Journal of bacteriology · 2008Article
- Flexibility of the cytoplasmic domain of the phototaxis transducer II from Natronomonas pharaonis.Journal of biophysics (Hindawi Publishing Corporation : Online) · 2008Article
- Laser-induced transient grating analysis of dynamics of interaction between sensory rhodopsin II D75N and the HtrII transducer.Biophysical journal · 2007Article
- Three strategically placed hydrogen-bonding residues convert a proton pump into a sensory receptor.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Rhodopsin-mediated photoreception in cryptophyte flagellates.Biophysical journal · 2005Article
- Time-resolved detection of sensory rhodopsin II-transducer interaction.Biophysical journal · 2004Article
- A eukaryotic BLUF domain mediates light-dependent gene expression in the purple bacterium Rhodobacter sphaeroides 2.4.1.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Chlamydomonas sensory rhodopsins A and B: cellular content and role in photophobic responses.Biophysical journal · 2004Article
- Moving fluid with bacterial carpets.Biophysical journal · 2004Article
- Side chains at the membrane-water interface modulate the signaling state of a transmembrane receptor.Biochemistry · 2004Article
- Archaeal protein kinases and protein phosphatases: insights from genomics and biochemistry.The Biochemical journal · 2003Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Halophilic archaea, such as Halobacterium salinarum and Natronobacterium pharaonis, alter their swimming behavior by phototaxis responses to changes in light intensity and color using visual pigment-like sensory rhodopsins (SRs). In N. pharaonis, SRII (NpSRII) mediates photorepellent responses through its transducer protein, NpHtrII. Here we report the expression of fusions of NpSRII and NpHtrII and fusion hybrids with eubacterial cytoplasmic domains and analyze their function in vivo in haloarchaea and in eubacteria. A fusion in which the C terminus of NpSRII is connected by a short flexible linker to NpHtrII is active in phototaxis signaling for H. salinarum, showing that the fusion does not inhibit functional receptor-transducer interactions. We replaced the cytoplasmic portions of this fusion protein with the cytoplasmic domains of Tar and Tsr, chemotaxis transducers from enteric eubacteria. Purification of the fusion protein from H. salinarum and Tar fusion chimera from Escherichia coli membranes shows that the proteins are not cleaved and exhibit absorption spectra characteristic of wild-type membranes. Their photochemical reaction cycles in H. salinarum and E. coli membranes, respectively, are similar to those of native NpSRII in N. pharaonis. These fusion chimeras mediate retinal-dependent phototaxis responses by Escherichia coli, establishing that the nine-helix membrane portion of the receptor-transducer complex is a modular functional unit able to signal in heterologous membranes. This result confirms a current model for SR-Htr signal transduction in which the Htr transducers are proposed to interact physically and functionally with their cognate sensory rhodopsins via helix-helix contacts between their transmembrane segments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.