ArticleJournal of bacteriology2001
Identification of the partitioning site within the repABC-type replicon of the composite Paracoccus versutus plasmid pTAV1.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 65 citations in OpenAlex.
- Differential Localization and Functional Specialization ofApplied and environmental microbiology · 2022Article
- In vivo creation of plasmid pCRT01 and its use for the construction of carotenoid-producing Paracoccus spp. strains that grow efficiently on industrial wastes.Microbial cell factories · 2020Article
- Multiple Megaplasmids Confer Extremely High Levels of Metal Tolerance inApplied and environmental microbiology · 2020Article
- Diversity and Horizontal Transfer of AntarcticGenes · 2019Article
- Characterization of the virome of Paracoccus spp. (Alphaproteobacteria) by combined in silico and in vivo approaches.Scientific reports · 2019Article
- Benefits and Drawbacks of Harboring Plasmid pP32BP2, Identified in Arctic Psychrophilic BacteriumInternational journal of molecular sciences · 2019Article
- Genomic Analysis ofInternational journal of molecular sciences · 2019Article
- Plasmids of PsychrotolerantFrontiers in microbiology · 2018Article
- Vitamin BScientific reports · 2017Article
- Genome-based insights into the resistome and mobilome of multidrug-resistant Aeromonas sp. ARM81 isolated from wastewater.Archives of microbiology · 2017Article
- Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria.BMC microbiology · 2016Article
- The repABC Plasmids with Quorum-Regulated Transfer Systems in Members of the Rhizobiales Divide into Two Structurally and Separately Evolving Groups.Genome biology and evolution · 2015Article
- Article
- Diversity and role of plasmids in adaptation of bacteria inhabiting the Lubin copper mine in Poland, an environment rich in heavy metals.Frontiers in microbiology · 2015Article
- Genome-guided insight into the methylotrophy of Paracoccus aminophilus JCM 7686.Frontiers in microbiology · 2015Article
- Molecular characterization of a novel temperate sinorhizobium bacteriophage, ФLM21, encoding DNA methyltransferase with CcrM-like specificity.Journal of virology · 2014Article
- Architecture and functions of a multipartite genome of the methylotrophic bacterium Paracoccus aminophilus JCM 7686, containing primary and secondary chromids.BMC genomics · 2014Article
- Mobility and generation of mosaic non-autonomous transposons by Tn3-derived inverted-repeat miniature elements (TIMEs).PloS one · 2014Article
- Plasmid diversity in arctic strains of Psychrobacter spp.Extremophiles : life under extreme conditions · 2013Article
- Characterization of Halomonas sp. ZM3 isolated from the Zelazny Most post-flotation waste reservoir, with a special focus on its mobile DNA.BMC microbiology · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The replicator region of composite plasmid pTAV1 of Paracoccus versutus (included in mini-replicon pTAV320) belongs to the family of repABC replicons commonly found in plasmids harbored by Agrobacterium and Rhizobium spp. The repABC replicons encode three genes clustered in an operon, which are involved in partitioning (repA and repB) and replication (repC). In order to localize the partitioning site of pTAV320, the two identified incompatibility determinants of this mini-replicon (inc1, located in the intergenic sequence between repB and repC; and inc2, situated downstream of the repC gene) were PCR amplified and used together with purified RepB fusion protein (homologous to the type B partitioning proteins binding to the partitioning sites) in an electrophoretic mobility shift assay. The protein bound only inc2, forming two complexes in a protein concentration-dependent manner. The inc2 region contains two long (14-bp) repeated sequences (R1 and R2). Disruption of these sequences completely eliminates RepB binding ability. R1 and R2 have sequence similarities with analogous repeats of another repABC replicon of plasmid pPAN1 of Paracoccus pantotrophus DSM 82.5 and with centromeric sequences of the Bacillus subtilis chromosome. Excess RepB protein resulted in destabilization of the inc2-containing plasmid in Escherichia coli. On the other hand, the inc2 region could stabilize another unstable replicon in P. versutus when RepA and RepB were delivered in trans, proving that this region has centromere-like activity. Thus, it was demonstrated that repA, repB, and inc2 constitute a functional system for active partitioning of pTAV320.
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