ArticleGenome research2001
Mouse BAC ends quality assessment and sequence analyses.
Article in Genome research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Improved protocols for BAC insert DNA isolation, BAC end sequencing and FISH for construction of BAC based physical map of genes on the chromosomes.Molecular biology reports · 2020Article
- pBACode: a random-barcode-based high-throughput approach for BAC paired-end sequencing and physical clone mapping.Nucleic acids research · 2017Article
- Generation of physical map contig-specific sequences.Frontiers in genetics · 2014Review
- HTS-PEG: a method for high throughput sequencing of the paired-ends of genomic libraries.PloS one · 2012Article
- Genetic mouse models to investigate cell cycle regulation.Transgenic research · 2009Article
- Genome evolution in Reptilia: in silico chicken mapping of 12,000 BAC-end sequences from two reptiles and a basal bird.BMC genomics · 2009Article
- Construction and sequence sampling of deep-coverage, large-insert BAC libraries for three model lepidopteran species.BMC genomics · 2009Article
- Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat.Plant molecular biology · 2009Article
- Comparative analysis of Alu repeats in primate genomes.Genome research · 2009Article
- A multiway analysis for identifying high integrity bovine BACs.BMC genomics · 2009Article
- Analysis of papaya BAC end sequences reveals first insights into the organization of a fruit tree genome.Molecular genetics and genomics : MGG · 2006Article
- Construction of a nurse shark (Ginglymostoma cirratum) bacterial artificial chromosome (BAC) library and a preliminary genome survey.BMC genomics · 2006Article
- Identification and chromosomal localization of repeat sequences through BAC end sequence analysis in Korean cattle.Journal of genetics · 2005Article
- Pooled genomic indexing of rhesus macaque.Genome research · 2005Article
- Contribution of Asian mouse subspecies Mus musculus molossinus to genomic constitution of strain C57BL/6J, as defined by BAC-end sequence-SNP analysis.Genome research · 2004Article
- Human, mouse, and rat genome large-scale rearrangements: stability versus speciation.Genome research · 2004Article
- Construction of a BAC library of Korean ginseng and initial analysis of BAC-end sequences.Molecular genetics and genomics : MGG · 2004Article
- A whole-genome mouse BAC microarray with 1-Mb resolution for analysis of DNA copy number changes by array comparative genomic hybridization.Genome research · 2004Article
- Concomitant reiterative BAC walking and fine genetic mapping enable physical map development for the broad-spectrum late blight resistance region, RB.Molecular genetics and genomics : MGG · 2003Article
- A cattle-human comparative map built with cattle BAC-ends and human genome sequence.Genome research · 2003Article
Corrections and comments
- Erratum issued
Authors and funding
18 authors.
Funding
Abstract
A large-scale BAC end-sequencing project at The Institute for Genomic Research (TIGR) has generated one of the most extensive sets of sequence markers for the mouse genome to date. With a sequencing success rate of >80%, an average read length of 485 bp, and ABI3700 capillary sequencers, we have generated 449,234 nonredundant mouse BAC end sequences (mBESs) with 218 Mb total from 257,318 clones from libraries RPCI-23 and RPCI-24, representing 15x clone coverage, 7% sequence coverage, and a marker every 7 kb across the genome. A total of 191,916 BACs have sequences from both ends providing 12x genome coverage. The average Q20 length is 406 bp and 84% of the bases have phred quality scores > or = 20. RPCI-24 mBESs have more Q20 bases and longer reads on average than RPCI-23 sequences. ABI3700 sequencers and the sample tracking system ensure that > 95% of mBESs are associated with the right clone identifiers. We have found that a significant fraction of mBESs contains L1 repeats and approximately 48% of the clones have both ends with > or = 100 bp contiguous unique Q20 bases. About 3% mBESs match ESTs and > 70% of matches were conserved between the mouse and the human or the rat. Approximately 0.1% mBESs contain STSs. About 0.2% mBESs match human finished sequences and > 70% of these sequences have EST hits. The analyses indicate that our high-quality mouse BAC end sequences will be a valuable resource to the community.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.