ArticleMolecular and cellular biology2001
TAF(II)170 interacts with the concave surface of TATA-binding protein to inhibit its DNA binding activity.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- BTAF1: a key regulator of DNA end resection and predictor of chemotherapy sensitivity in gastric cancer.Cell death and differentiation · 2026Article
- De Novo Heterozygous POLR2A Variants Cause a Neurodevelopmental Syndrome with Profound Infantile-Onset Hypotonia.American journal of human genetics · 2019Article
- Co-translational assembly of mammalian nuclear multisubunit complexes.Nature communications · 2019Article
- Quantitative Proteomics of the SMAD (Suppressor of Mothers against Decapentaplegic) Transcription Factor Family Identifies Importin 5 as a Bone Morphogenic Protein Receptor SMAD-specific Importin.The Journal of biological chemistry · 2016Article
- Molecular Mechanism of Mot1, a TATA-binding Protein (TBP)-DNA Dissociating Enzyme.The Journal of biological chemistry · 2016Article
- Tight cooperation between Mot1p and NC2β in regulating genome-wide transcription, repression of transcription following heat shock induction and genetic interaction with SAGA.Nucleic acids research · 2012Article
- One small step for Mot1; one giant leap for other Swi2/Snf2 enzymes?Biochimica et biophysica acta · 2011Review
- Structure and mechanism of the Swi2/Snf2 remodeller Mot1 in complex with its substrate TBP.Nature · 2011Article
- TBP-related factors: a paradigm of diversity in transcription initiation.Cell & bioscience · 2011Article
- A proteomics analysis of yeast Mot1p protein-protein associations: insights into mechanism.Molecular & cellular proteomics : MCP · 2008Article
- Function and structural organization of Mot1 bound to a natural target promoter.The Journal of biological chemistry · 2008Article
- The transcriptional repressor activator protein Rap1p is a direct regulator of TATA-binding protein.The Journal of biological chemistry · 2008Article
- Yeast two-hybrid map of Arabidopsis TFIID.Plant molecular biology · 2007Article
- Snf2/Swi2-related ATPase Mot1 drives displacement of TATA-binding protein by gripping DNA.The EMBO journal · 2006Article
- Mutational analysis of BTAF1-TBP interaction: BTAF1 can rescue DNA-binding defective TBP mutants.Nucleic acids research · 2005Article
- NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TATA-binding protein.Molecular and cellular biology · 2004Article
- High-affinity DNA binding by a Mot1p-TBP complex: implications for TAF-independent transcription.The EMBO journal · 2003Article
- Structural and functional analysis of mutations along the crystallographic dimer interface of the yeast TATA binding protein.Molecular and cellular biology · 2003Article
- A TATA binding protein mutant with increased affinity for DNA directs transcription from a reversed TATA sequence in vivo.Molecular and cellular biology · 2002Article
- Mot1p is essential for TBP recruitment to selected promoters during in vivo gene activation.The EMBO journal · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human RNA polymerase II transcription factor B-TFIID consists of TATA-binding protein (TBP) and the TBP-associated factor (TAF) TAF(II)170 and can rapidly redistribute over promoter DNA. Here we report the identification of human TBP-binding regions in human TAF(II)170. We have defined the TBP interaction domain of TAF(II)170 within three amino-terminal regions: residues 2 to 137, 290 to 381, and 380 to 460. Each region contains a pair of Huntington-elongation-A subunit-Tor repeats and exhibits species-specific interactions with TBP family members. Remarkably, the altered-specificity TBP mutant (TBP(AS)) containing a triple mutation in the concave surface is defective for binding the TAF(II)170 amino-terminal region of residues 1 to 504. Furthermore, within this region the TAF(II)170 residues 290 to 381 can inhibit the interaction between Drosophila TAF(II)230 (residues 2 to 81) and TBP through competition for the concave surface of TBP. Biochemical analyses of TBP binding to the TATA box indicated that TAF(II)170 region 290-381 inhibits TBP-DNA complex formation. Importantly, the TBP(AS) mutant is less sensitive to TAF(II)170 inhibition. Collectively, our results support a mechanism in which TAF(II)170 induces high-mobility DNA binding by TBP through reversible interactions with its concave DNA binding surface.
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