ArticleMolecular and cellular biology2001
Mechanism of poly(A) signal transduction to RNA polymerase II in vitro.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Human TRiC complex purified from HeLa cells contains all eight CCT subunits and is active in vitro.Cell stress & chaperones · 2013Article
- RNA polymerase II pausing downstream of core histone genes is different from genes producing polyadenylated transcripts.PloS one · 2012Article
- Coupled RNA polymerase II transcription and 3' end formation with yeast whole-cell extracts.RNA (New York, N.Y.) · 2010Article
- Poly(A) signal-dependent degradation of unprocessed nascent transcripts accompanies poly(A) signal-dependent transcriptional pausing in vitro.RNA (New York, N.Y.) · 2010Article
- Torpedo nuclease Rat1 is insufficient to terminate RNA polymerase II in vitro.The Journal of biological chemistry · 2009Article
- Polyadenylation releases mRNA from RNA polymerase II in a process that is licensed by splicing.RNA (New York, N.Y.) · 2009Article
- Functional coupling of last-intron splicing and 3'-end processing to transcription in vitro: the poly(A) signal couples to splicing before committing to cleavage.Molecular and cellular biology · 2008Article
- The conserved AAUAAA hexamer of the poly(A) signal can act alone to trigger a stable decrease in RNA polymerase II transcription velocity.RNA (New York, N.Y.) · 2006Article
- Npl3 is an antagonist of mRNA 3' end formation by RNA polymerase II.The EMBO journal · 2005Article
- Strong polyadenylation and weak pausing combine to cause efficient termination of transcription in the human Ggamma-globin gene.Molecular and cellular biology · 2005Article
- Identification of cis elements directing termination of yeast nonpolyadenylated snoRNA transcripts.Molecular and cellular biology · 2004Article
- Functions for S. cerevisiae Swd2p in 3' end formation of specific mRNAs and snoRNAs and global histone 3 lysine 4 methylation.RNA (New York, N.Y.) · 2004Article
- Transitions in RNA polymerase II elongation complexes at the 3' ends of genes.The EMBO journal · 2004Article
- The role of the yeast cleavage and polyadenylation factor subunit Ydh1p/Cft2p in pre-mRNA 3'-end formation.Nucleic acids research · 2003Article
- Independent functions of yeast Pcf11p in pre-mRNA 3' end processing and in transcription termination.The EMBO journal · 2003Article
- Functional interactions between the transcription and mRNA 3' end processing machineries mediated by Ssu72 and Sub1.Genes & development · 2003Article
- Yhh1p/Cft1p directly links poly(A) site recognition and RNA polymerase II transcription termination.The EMBO journal · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
Termination of transcription by RNA polymerase II usually requires the presence of a functional poly(A) site. How the poly(A) site signals its presence to the polymerase is unknown. All models assume that the signal is generated after the poly(A) site has been extruded from the polymerase, but this has never been tested experimentally. It is also widely accepted that a "pause" element in the DNA stops the polymerase and that cleavage at the poly(A) site then signals termination. These ideas also have never been tested. The lack of any direct tests of the poly(A) signaling mechanism reflects a lack of success in reproducing the poly(A) signaling phenomenon in vitro. Here we describe a cell-free transcription elongation assay that faithfully recapitulates poly(A) signaling in a crude nuclear extract. The assay requires the use of citrate, an inhibitor of RNA polymerase II carboxyl-terminal domain phosphorylation. Using this assay we show the following. (i) Wild-type but not mutant poly(A) signals instruct the polymerase to stop transcription on downstream DNA in a manner that parallels true transcription termination in vivo. (ii) Transcription stops without the need of downstream elements in the DNA. (iii) cis-antisense inhibition blocks signal transduction, indicating that the signal to stop transcription is generated following extrusion of the poly(A) site from the polymerase. (iv) Signaling can be uncoupled from processing, demonstrating that signaling does not require cleavage at the poly(A) site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.