ArticleMolecular and cellular biology2001
Common regulation of growth arrest and differentiation of osteoblasts by helix-loop-helix factors.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 120 citations in OpenAlex.
- Cellular functions and molecular mechanisms of ubiquitination in osteosarcoma.Frontiers in oncology · 2022Pooled it
- Epithelial-to-mesenchymal transition transcription factors: New strategies for mesenchymal tissue regeneration.Cytokine & growth factor reviews · 2025Review
- Ubiquitin-specific peptidases: Players in bone metabolism.Cell proliferation · 2023Review
- Deubiquitinating Enzyme USP7 Is Required for Self-Renewal and Multipotency of Human Bone Marrow-Derived Mesenchymal Stromal Cells.International journal of molecular sciences · 2022Article
- Role of Organic Cation Transporter 2 in Autophagy Induced by Platinum Derivatives.International journal of molecular sciences · 2022Article
- MonoallelicFrontiers in immunology · 2022Article
- AKT-mediated phosphorylation of TWIST1 is essential for breast cancer cell metastasis.Turkish journal of biology = Turk biyoloji dergisi · 2020Article
- Twist1 Inactivation in Dmp1-Expressing Cells Increases Bone Mass but Does Not Affect the Anabolic Response to Sclerostin Neutralization.International journal of molecular sciences · 2019Article
- Twist1 Suppresses Cementoblast Differentiation.Dentistry journal · 2018Article
- Molecular dissection of germline chromothripsis in a developmental context using patient-derived iPS cells.Genome medicine · 2017Article
- Phosphorylation Regulates Id2 Degradation and Mediates the Proliferation of Neural Precursor Cells.Stem cells (Dayton, Ohio) · 2016Article
- Serum Deprivation-Induced Human GM3 Synthase (hST3Gal V) Gene Expression Is Mediated by Runx2 in Human Osteoblastic MG-63 Cells.International journal of molecular sciences · 2015Article
- Functional Analysis of Two Novel Mutations in TWIST1 Protein Motifs Found in Ventricular Septal Defect Patients.Pediatric cardiology · 2015Article
- Krüppel-like factor 2 represses transcription of the telomerase catalytic subunit human telomerase reverse transcriptase (hTERT) in human T cells.The Journal of biological chemistry · 2015Article
- The ID proteins: master regulators of cancer stem cells and tumour aggressiveness.Nature reviews. Cancer · 2014Review
- TWIST interacts with endothelin-1/endothelin A receptor signaling in osteosarcoma cell survival against cisplatin.Oncology letters · 2013Article
- E proteins regulate osteoclast maturation and survival.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2012Article
- Computational modeling of the bHLH domain of the transcription factor TWIST1 and R118C, S144R and K145E mutants.BMC bioinformatics · 2012Article
- Tbx1 regulates oral epithelial adhesion and palatal development.Human molecular genetics · 2012Article
- Mmp15 is a direct target of Snai1 during endothelial to mesenchymal transformation and endocardial cushion development.Developmental biology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cellular differentiation entails the coordination of cell cycle arrest and tissue-specific gene expression. We investigated the involvement of basic helix-loop-helix (bHLH) factors in differentiation of osteoblasts using the human osteoblastic cell line MG63. Serum starvation induced growth arrest at G1 phase, accompanied by expression of cyclin-dependent kinase inhibitor p21(WAF1/Cip1). Reporter assays with the p21 gene promoter demonstrated that the combination of E2A (E12 or E47) and coactivator CBP was responsible for p21 induction independent of p53. Twist inhibited E2A-CBP-dependent activation of the exogenous and endogenous p21 promoters. Ids similarly inhibited the exogenously transfected p21 promoter; however less antagonistic effect on the endogenous p21 promoter was observed. Twist was predominantly present in nuclei in MG63 cells growing in complete medium, while it localized mainly in the cytoplasm after serum starvation. The fibroblast growth factor receptor 3 gene (FGFR3), which generates signals leading to differentiation of osteoblasts, was found to be controlled by the same transcriptional regulation as the p21 gene. E2A and Twist influenced alkaline phosphatase expression, a consensus marker of osteoblast differentiation. Expression of E2A and FGFR3 was seen at the location of osteoblast differentiation in the calvaria of mouse embryos, implicating bHLH molecules in physiological osteoblast differentiation. These results demonstrate that a common regulatory system is involved in at least two distinct steps in osteoblastic differentiation. Our results also provide the molecular basis of Saethre-Chotzen syndrome, caused by mutations of the TWIST and FGFR3 genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.