ArticleMolecular and cellular biology2001
Analysis of TAF90 mutants displaying allele-specific and broad defects in transcription.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Fine mapping of QTL conferring Cercospora leaf spot disease resistance in mungbean revealed TAF5 as candidate gene for the resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- The SAGA chromatin-modifying complex: the sum of its parts is greater than the whole.Genes & development · 2020Review
- Beyond Histones: New Substrate Proteins of Lysine Deacetylases in Arabidopsis Nuclei.Frontiers in plant science · 2018Article
- Article
- Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex.The EMBO journal · 2014Article
- Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation.The EMBO journal · 2011Article
- Direct transactivator-transcription factor IID (TFIID) contacts drive yeast ribosomal protein gene transcription.The Journal of biological chemistry · 2010Article
- Recent advances in understanding the structure and function of general transcription factor TFIID.Cellular and molecular life sciences : CMLS · 2009Review
- Dissection of coactivator requirement at RNR3 reveals unexpected contributions from TFIID and SAGA.The Journal of biological chemistry · 2008Article
- The essential gene wda encodes a WD40 repeat subunit of Drosophila SAGA required for histone H3 acetylation.Molecular and cellular biology · 2006Article
- Article
- Computational verification of protein-protein interactions by orthologous co-expression.BMC bioinformatics · 2005Article
- Mapping key functional sites within yeast TFIID.The EMBO journal · 2004Article
- Systematic analysis of essential yeast TAFs in genome-wide transcription and preinitiation complex assembly.The EMBO journal · 2003Article
- Protein-protein interaction map for yeast TFIID.Nucleic acids research · 2003Article
- SWI/SNF-dependent chromatin remodeling of RNR3 requires TAF(II)s and the general transcription machinery.Genes & development · 2003Article
- Identification of histone H4-like TAF in Schizosaccharomyces pombe as a protein that interacts with WD repeat-containing TAF.Nucleic acids research · 2002Article
- Distinct mutations in yeast TAF(II)25 differentially affect the composition of TFIID and SAGA complexes as well as global gene expression patterns.Molecular and cellular biology · 2002Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Yeast TAF90p is a component of at least two transcription regulatory complexes, the general transcription factor TFIID and the Spt-Ada-Gcn5 histone acetyltransferase complex (SAGA). Broad transcription defects have been observed in mutants of other TAF(II)s shared by TFIID and SAGA but not in the only two TAF90 mutants isolated to date. Given that the numbers of mutants analyzed thus far are small, we isolated and characterized 11 temperature-sensitive mutants of TAF90 and analyzed their effects on transcription and integrity of the TFIID and SAGA complexes. We found that the mutants displayed a variety of allele-specific defects in their ability to support transcription and maintain the structure of the TFIID and SAGA complexes. Sequencing of the alleles revealed that all have mutations corresponding to the C terminus of the protein, with most clustering within the conserved WD40 repeats; thus, the C terminus of TAF90p is required for its incorporation into TFIID and function in SAGA. Significantly, inactivation of one allele, taf90-20, caused the dramatic reduction in the levels of total mRNA and most specific transcripts analyzed. Analysis of the structure and/or activity of both TAF90p-containing complexes revealed that this allele is the most disruptive of all. Our analysis defines the requirement for the WD40 repeats in preserving TFIID and SAGA function, demonstrates that the defects associated with distinct mutations in TAF90 vary considerably, and indicates that TAF90 can be classified as a gene required for the transcription of a large number of genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.