ArticleThe Biochemical journal2001
Endothelin-1 induces phosphorylation of GATA-4 transcription factor in the HL-1 atrial-muscle cell line.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 61 citations in OpenAlex.
- Impact of High Altitude on Cardiovascular Health: Current Perspectives.Vascular health and risk management · 2021Review
- Experimental models of cardiac physiology and pathology.Heart failure reviews · 2019Review
- Model systems for cardiovascular regenerative biology.Cold Spring Harbor perspectives in medicine · 2013Review
- Suppression of Induced microRNA-15b Prevents Rapid Loss of Cardiac Function in a Dicer Depleted Model of Cardiac Dysfunction.PloS one · 2013Article
- Transcription factors in heart: promising therapeutic targets in cardiac hypertrophy.Current cardiology reviews · 2011Review
- Cell signaling pathways for the regulation of GATA4 transcription factor: Implications for cell growth and apoptosis.Cellular signalling · 2011Article
- Mechanism of anthracycline-mediated down-regulation of GATA4 in the heart.Cardiovascular research · 2011Article
- Pulmonary hypertension-induced GATA4 activation in the right ventricle.Hypertension (Dallas, Tex. : 1979) · 2010Article
- Critical role of nuclear calcium/calmodulin-dependent protein kinase IIdeltaB in cardiomyocyte survival in cardiomyopathy.The Journal of biological chemistry · 2009Article
- Heme oxygenase-1 inhibits pro-oxidant induced hypertrophy in HL-1 cardiomyocytes.Experimental biology and medicine (Maywood, N.J.) · 2009Article
- Re-employment of developmental transcription factors in adult heart disease.Seminars in cell & developmental biology · 2007Review
- The calcium-binding activity of a vacuole-associated, dehydrin-like protein is regulated by phosphorylation.Plant physiology · 2002Article
- Cell-specific phosphorylation of Zfhep transcription factor.Biochemical and biophysical research communications · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
The transcription factor GATA-4 plays a central role in the regulation of cardiac-muscle gene transcription. The present study demonstrates that endothelin-1 (ET-1) induces GATA-4 activation and phosphorylation. The treatment of HL-1 adult mouse atrial-muscle cells with ET-1 (30 nM) caused a rapid increase in the DNA binding activity of GATA-4 within 3 min. The activation was associated with an upward mobility shift of the GATA-4 band on native PAGE in an electrophoretic- mobility-shift assay. The upward shift of the GATA-4 band also occurred on SDS/PAGE as monitored by immunoblotting. The in vitro treatment of nuclear extracts with lambda-protein phosphatase abolished the upward shift, indicating that GATA-4 was phosphorylated. ET-1 activated the p44/42 mitogen-activated protein kinase (MAPK) and the MAPK kinase (MEK) within 3 min, and PD98059 (a specific inhibitor of MEK) abolished the ET-1-induced GATA-4 phosphorylation. PMA also caused the rapid activation of MAPK and the phosphorylation of GATA-4. In contrast, the activation of MAPK by phenylephrine or H(2)O(2) was weak and did not lead to GATA-4 phosphorylation. Thus ET-1 induces a GATA-4 phosphorylation by activating a MEK-MAPK pathway.
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