ArticleJournal of virology2001
Role for the adenovirus IVa2 protein in packaging of viral DNA.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 91 citations in OpenAlex.
- Phosphorylation regulates viral biomolecular condensates to promote infectious progeny production.The EMBO journal · 2024Article
- PML Alternative Splice Products Differentially Regulate HAdV Productive Infection.Microbiology spectrum · 2022Article
- RANBP2 and USP9x regulate nuclear import of adenovirus minor coat protein IIIa.PLoS pathogens · 2022Article
- Genomics-based re-examination of the taxonomy and phylogeny of human and simian Mastadenoviruses: an evolving whole genomes approach, revealing putative zoonosis, anthroponosis, and amphizoonosis.Cladistics : the international journal of the Willi Hennig Society · 2020Article
- Adenoviral E4 34K protein interacts with virus packaging components and may serve as the putative portal.Scientific reports · 2017Article
- Article
- Components of Adenovirus Genome Packaging.Frontiers in microbiology · 2016Review
- Structures of Adenovirus Incomplete Particles Clarify Capsid Architecture and Show Maturation Changes of Packaging Protein L1 52/55k.Journal of virology · 2015Article
- Adenoviral L4 33K forms ring-like oligomers and stimulates ATPase activity of IVa2: implications in viral genome packaging.Frontiers in microbiology · 2015Article
- Impact of adenovirus life cycle progression on the generation of canine helper-dependent vectors.Gene therapy · 2015Article
- Processing of the l1 52/55k protein by the adenovirus protease: a new substrate and new insights into virion maturation.Journal of virology · 2014Article
- Real-time sensing and discrimination of single chemicals using the channel of phi29 DNA packaging nanomotor.ACS nano · 2012Article
- Adenovirus structural protein IIIa is involved in the serotype specificity of viral DNA packaging.Journal of virology · 2011Article
- Engineering of the fluorescent-energy-conversion arm of phi29 DNA packaging motor for single-molecule studies.Small (Weinheim an der Bergstrasse, Germany) · 2009Article
- Cidofovir inhibits genome encapsidation and affects morphogenesis during the replication of vaccinia virus.Journal of virology · 2009Article
- Genomic analyses of recombinant adenovirus type 11a in China.Journal of clinical microbiology · 2009Article
- Accurate single-day titration of adenovirus vectors based on equivalence of protein VII nuclear dots and infectious particles.Journal of virological methods · 2009Article
- Adenovirus serotype 5 L4-22K and L4-33K proteins have distinct functions in regulating late gene expression.Journal of virology · 2009Article
- Article
- Adenovirus IVa2 protein binds ATP.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Although it has been demonstrated that the adenovirus IVa2 protein binds to the packaging domains on the viral chromosome and interacts with the viral L1 52/55-kDa protein, which is required for viral DNA packaging, there has been no direct evidence demonstrating that the IVa2 protein is involved in DNA packaging. To understand in greater detail the DNA packaging mechanisms of adenovirus, we have asked whether DNA packaging is serotype or subgroup specific. We found that Ad7 (subgroup B), Ad12 (subgroup A), and Ad17 (subgroup D) cannot complement the defect of an Ad5 (subgroup C) mutant, pm8001, which does not package its DNA due to a mutation in the L1 52/55-kDa gene. This indicates that the DNA packaging systems of different serotypes cannot interact productively with Ad5 DNA. Based on this, a chimeric virus containing the Ad7 genome except for the inverted terminal repeats and packaging sequence from Ad5 was constructed. This chimeric virus replicates its DNA and synthesizes Ad7 proteins, but it cannot package its DNA in 293 cells or 293 cells expressing the Ad5 L1 52/55-kDa protein. However, this chimeric virus packages its DNA in 293 cells expressing the Ad5 IVa2 protein. These results indicate that the IVa2 protein plays a role in viral DNA packaging and that its function is serotype specific. Since this chimeric virus cannot package its own DNA, but produces all the components for packaging Ad7 DNA, it may be a more suitable helper virus for the growth of Ad7 gutted vectors for gene transfer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.