ArticleJournal of virology2001
Rep-dependent initiation of adeno-associated virus type 2 DNA replication by a herpes simplex virus type 1 replication complex in a reconstituted system.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed.
- Expression of Viral DNA Polymerase in Synthetic Recombinant Adeno-Associated Virus Producer Cell Line Enhances Full Particle Productivity.Biotechnology and bioengineering · 2025Article
- AAV2 can replicate its DNA by a rolling hairpin or rolling circle mechanism, depending on the helper virus.Journal of virology · 2024Article
- Human Bocavirus 1 NP1 acts as an ssDNA-binding protein to help AAV2 DNA replication and cooperates with RPA to regulate AAV2 capsid expression.Journal of virology · 2024Article
- Herpes simplex virus co-infection facilitates rolling circle replication of the adeno-associated virus genome.PLoS pathogens · 2021Article
- Micro-dystrophin AAV Vectors Made by Transient Transfection and Herpesvirus System Are Equally Potent in Treating mdx Mouse Muscle Disease.Molecular therapy. Methods & clinical development · 2020Article
- Review
- Adeno-Associated Virus Genome Interactions Important for Vector Production and Transduction.Human gene therapy · 2020Review
- Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1.Journal of virology · 2017Article
- Generation of infectious recombinant Adeno-associated virus in Saccharomyces cerevisiae.PloS one · 2017Article
- Adeno-Associated Virus Type 2 Rep68 Can Bind to Consensus Rep-Binding Sites on the Herpes Simplex Virus 1 Genome.Journal of virology · 2015Article
- Human bocavirus: lessons learned to date.Pathogens (Basel, Switzerland) · 2013Review
- Viral and Cellular Components of AAV2 Replication Compartments.The open virology journal · 2013Article
- Article
- Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments.Journal of virology · 2010Article
- Herpes simplex virus type 1/adeno-associated virus hybrid vectors.The open virology journal · 2010Article
- Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities.Journal of virology · 2010Article
- Transcriptional analysis of the adeno-associated virus integration site.Journal of virology · 2009Article
- Large-scale adeno-associated viral vector production using a herpesvirus-based system enables manufacturing for clinical studies.Human gene therapy · 2009Review
- Definition of herpes simplex virus type 1 helper activities for adeno-associated virus early replication events.PLoS pathogens · 2009Article
- Complete in vitro reconstitution of adeno-associated virus DNA replication requires the minichromosome maintenance complex proteins.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Productive infection by adeno-associated virus type 2 (AAV) requires coinfection with a helper virus, e.g., adenovirus or herpesviruses. In the case of adenovirus coinfection, the replication machinery of the host cell performs AAV DNA replication. In contrast, it has been proposed that the herpesvirus replication machinery might replicate AAV DNA. To investigate this question, we have attempted to reconstitute AAV DNA replication in vitro using purified herpes simplex virus type 1 (HSV-1) replication proteins. We show that the HSV-1 UL5, UL8, UL29, UL30, UL42, and UL52 gene products along with the AAV Rep68 protein are sufficient to initiate replication on duplex DNA containing the AAV origins of replication, resulting in products several hundred nucleotides in length. Initiation can occur also on templates containing only a Rep binding site and a terminal resolution site. We further demonstrate that initiation of DNA synthesis can take place with a subset of these factors: Rep68 and the UL29, UL30, and UL42 gene products. Since the HSV polymerase and its accessory factor (the products of the UL30 and UL42 genes) are unable to efficiently perform synthesis by strand displacement, it is likely that in addition to creating a hairpin primer, the AAV Rep protein also acts as a helicase for DNA synthesis. The single-strand DNA binding protein (the UL29 gene product) presumably prevents reannealing of complementary strands. These results suggest that AAV can use the HSV replication apparatus to replicate its DNA. In addition, they may provide a first step for the development of a fully reconstituted AAV replication assay.
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