ArticleThe Journal of experimental medicine2001
The upstream enhancer is necessary and sufficient for the expression of the pre-T cell receptor alpha gene in immature T lymphocytes.
Article in The Journal of experimental medicine, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 53 citations in OpenAlex.
- A critical regulator of Bcl2 revealed by systematic transcript discovery of lncRNAs associated with T-cell differentiation.Scientific reports · 2019Article
- The establishment of B versus T cell identity.Trends in immunology · 2014Review
- Transcriptional competence and the active marking of tissue-specific enhancers by defined transcription factors in embryonic and induced pluripotent stem cells.Genes & development · 2009Article
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- BAC transgenic expression efficiency: bicistronic versus ATG-fusion strategies.Genesis (New York, N.Y. : 2000) · 2007Article
- Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Pioneer factor interactions and unmethylated CpG dinucleotides mark silent tissue-specific enhancers in embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- BAC to immunology--bacterial artificial chromosome-mediated transgenesis for targeting of immune cells.Immunology · 2007Review
- The Bloom's syndrome helicase is critical for development and function of the alphabeta T-cell lineage.Molecular and cellular biology · 2007Article
- Production of green fluorescent protein transgenic embryonic stem cells using the GENSAT bacterial artificial chromosome library.Stem cells (Dayton, Ohio) · 2007Article
- E proteins and Notch signaling cooperate to promote T cell lineage specification and commitment.The Journal of experimental medicine · 2006Article
- v-Myb mediates cooperation of a cell-specific enhancer with the mim-1 promoter.Molecular and cellular biology · 2005Article
- Article
- E2A proteins enforce a proliferation checkpoint in developing thymocytes.The EMBO journal · 2004Article
- Disruption of pre-TCR expression accelerates lymphomagenesis in E2A-deficient mice.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Identification and characterization of the Myb-inducible promoter of the chicken adenosine receptor 2B gene.Oncogene · 2002Article
- Article
- Combined expression of pTalpha and Notch3 in T cell leukemia identifies the requirement of preTCR for leukemogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway.Genes & development · 2002Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of the pre-T cell receptor alpha (pTa) gene occurs exclusively in immature T lymphocytes and is regulated by poorly defined mechanisms. We have analyzed the role of the upstream enhancer in pTa expression using conventional and bacterial artificial chromosome (BAC) reporter transgenes. The deletion of the enhancer completely abolished the expression of pTa BAC reporter in transgenic mice. Conversely, the combination of pTa enhancer and promoter targeted transgenes specifically to immature thymocytes, recapitulating the expression pattern of pTa. The core enhancer is conserved between mice and humans and contains a critical binding site for the transcription factor c-Myb. We also show that pTa promoter contains a conserved tandem E box site activated by E protein, HEB. These data establish the enhancer as a critical element regulating pTa gene expression and identify additional targets for c-Myb and E proteins in T cell development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.