ArticleThe Journal of cell biology2001
Pivotal role of VASP in Arp2/3 complex-mediated actin nucleation, actin branch-formation, and Listeria monocytogenes motility.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 132 citations in OpenAlex.
- Proteome from patients with metabolic syndrome is regulated by quantity and quality of dietary lipids.BMC genomics · 2015Trial
- Force-activated zyxin assemblies coordinate actin nucleation and crosslinking to orchestrate stress fiber repair.bioRxiv : the preprint server for biology · 2024Article
- The Role of Vasodilator-stimulated Phosphoproteins in the Development of Malignant Tumors.Current cancer drug targets · 2024Review
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- Cooperation of Striatin 3 and MAP4K4 promotes growth and tissue invasion.Communications biology · 2022Article
- Review
- Article
- Fe65: A Scaffolding Protein of Actin Regulators.Cells · 2021Review
- Capping protein is dispensable for polarized actin network growth and actin-based motility.The Journal of biological chemistry · 2020Article
- Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion.eLife · 2020Article
- AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting.Acta physiologica (Oxford, England) · 2020Article
- Article
- Actin-based motility allows Listeria monocytogenes to avoid autophagy in the macrophage cytosol.Cellular microbiology · 2018Article
- Article
- VASP regulates leukocyte infiltration, polarization, and vascular repair after ischemia.The Journal of cell biology · 2018Article
- CRMP-1 enhances EVL-mediated actin elongation to build lamellipodia and the actin cortex.The Journal of cell biology · 2017Article
- Actin-based motility and cell-to-cell spread of bacterial pathogens.Current opinion in microbiology · 2017Review
- Vasodilator-Stimulated Phosphoprotein Activity Is Required for Coxiella burnetii Growth in Human Macrophages.PLoS pathogens · 2016Article
- Fascin Rigidity and L-plastin Flexibility Cooperate in Cancer Cell Invadopodia and Filopodia.The Journal of biological chemistry · 2016Article
- Profilin Interaction with Actin Filament Barbed End Controls Dynamic Instability, Capping, Branching, and Motility.Developmental cell · 2016Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The Listeria monocytogenes ActA protein mediates actin-based motility by recruiting and stimulating the Arp2/3 complex. In vitro, the actin monomer-binding region of ActA is critical for stimulating Arp2/3-dependent actin nucleation; however, this region is dispensable for actin-based motility in cells. Here, we provide genetic and biochemical evidence that vasodilator-stimulated phosphoprotein (VASP) recruitment by ActA can bypass defects in actin monomer-binding. Furthermore, purified VASP enhances the actin-nucleating activity of wild-type ActA and the Arp2/3 complex while also reducing the frequency of actin branch formation. These data suggest that ActA stimulates the Arp2/3 complex by both VASP-dependent and -independent mechanisms that generate distinct populations of actin filaments in the comet tails of L. monocytogenes. The ability of VASP to contribute to actin filament nucleation and to regulate actin filament architecture highlights the central role of VASP in actin-based motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.