ArticleInflammation2001
Induction of nitric oxide synthesis by probiotic Lactobacillus rhamnosus GG in J774 macrophages and human T84 intestinal epithelial cells.
Article in Inflammation, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 97 citations in OpenAlex.
- Effects of probiotic on intestinal mucosa of patients with ulcerative colitis.World journal of gastroenterology · 2004Trial
- Effects and mechanisms of probiotics supplement on hypertension.Journal of hypertension · 2026Review
- Therapeutic potential of heat-killed Lactobacillus reuteri against bile acid-induced male reproductive toxicity.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Studies of the Impact of the Bifidobacterium Species on Inducible Nitric Oxide Synthase Expression and Nitric Oxide Production in Murine Macrophages of the BMDM Cell Line.Probiotics and antimicrobial proteins · 2024Article
- Blends of Organic Acids Are Weaponizing the Host iNOS and Nitric Oxide to Reduce Infection ofAntioxidants (Basel, Switzerland) · 2024Article
- Characterization of Lactic Acid Bacteria from Fermented Fish (pla-paeng-daeng) and Their Cholesterol-lowering and Immunomodulatory Effects.Microbes and environments · 2023Article
- Cell-Free Supernatant fromMolecules (Basel, Switzerland) · 2022Article
- Distribution, cholesterol-lowering and immunomodulation effects of lactic acid bacteria from fermented mussel (Heliyon · 2022Article
- Psychobiotics: the Influence of Gut Microbiota on the Gut-Brain Axis in Neurological Disorders.Journal of molecular neuroscience : MN · 2022Article
- A Review of the Effects and Production of Spore-Forming Probiotics for Poultry.Animals : an open access journal from MDPI · 2021Review
- Antioxidant activity and short-chain fatty acid production of lactic acid bacteria isolated from Korean individuals and fermented foods.3 Biotech · 2021Article
- Interventions of natural and synthetic agents in inflammatory bowel disease, modulation of nitric oxide pathways.World journal of gastroenterology · 2020Review
- Para-probiotics for Preterm Neonates-The Next Frontier.Nutrients · 2018Review
- Current Perspectives on Antihypertensive Probiotics.Probiotics and antimicrobial proteins · 2017Review
- Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium.World journal of microbiology & biotechnology · 2017Article
- Article
- Mature Biofilm Degradation by Potential Probiotics: Aggregatibacter actinomycetemcomitans versus Lactobacillus spp.PloS one · 2016Article
- Biodiversity of Intestinal Lactic Acid Bacteria in the Healthy Population.Advances in experimental medicine and biology · 2016Review
- Kefir treatment ameliorates dextran sulfate sodium-induced colitis in rats.World journal of gastroenterology · 2015Article
- Effects of strains of Lactococcus lactis on the production of nitric oxide and cytokines in murine macrophages.Inflammation · 2014Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUNDS AND
aimsProbiotic Lactobacillus rhamnosus GG (LGG) has proved to be beneficial in the treatment of viral- and antibiotic-associated diarrhea but the mechanisms of action remain unknown. Nitric oxide (NO) is involved in the protective mechanisms in the gastrointestinal tract and may contribute to some of the beneficial effects of probiotics. The aim of the present study was to investigate if induction of NO synthesis is involved in the cellular actions of LGG.
methodsNO synthesis and its regulation were measured in cultures of J774 murine macrophages and human T84 colon epithelial cells. NO production was measured as its metabolite nitrite accumulated into the culture medium. Inducible nitric oxide synthase (iNOS) protein and iNOS mRNA were detected by Western blot and RT-PCR, respectively.
resultsIn J774 macrophages, LGG induced a low level production of NO in the presence of gamma interferon (IFNgamma) and it was inhibited by NOS inhibitors, cycloheximide and by a NF-kappa B inhibitor pyrrolidinedithiocarbamate. Accordingly, LGG and IFNgamma-stimulation increased iNOS mRNA and protein levels. T84 cells produced NO in response to LGG when first primed with a combination of IL-1beta, TNFalpha and IFNgamma. Lipoteichoic acid (LA), an antigenic structure in gram-positive bacteria, also induced NO formation in J774 cells in the presence of IFNgamma suggesting that LA may be the active component in LGG.
conclusionsLGG induces NO production in J774 macrophages and in human T84 colon epithelial cells through induction of iNOS by a mechanism involving activation of transcription factor NF-kappaB. Induction of iNOS and low-level synthesis of NO may be involved in the protective actions of LGG in the gastrointestinal tract.
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