ArticleJournal of clinical pathology2001
High quality RNA isolation from tumours with low cellularity and high extracellular matrix component for cDNA microarrays: application to chondrosarcoma.
Article in Journal of clinical pathology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 93 citations in OpenAlex.
- RNA Extraction from Cartilage: Issues, Methods, Tips.International journal of molecular sciences · 2023Review
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- The role of Dermcidin isoform-2 in the occurrence and severity of Diabetes.Scientific reports · 2017Article
- A Laboratory Methodology for Dual RNA-Sequencing of Bacteria and their Host CellsFrontiers in microbiology · 2017Article
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- Expression of osteoprotegerin, RNAK and RANKL genes in femoral head avascular necrosis and related signaling pathway.International journal of clinical and experimental pathology · 2015Article
- The Effect of Tumor Microenvironment on Autophagy and Sensitivity to Targeted Therapy in EGFR-Mutated Lung Adenocarcinoma.Journal of Cancer · 2015Article
- The effects of protein solubility on the RNA Integrity Number (RIN) for recombinantBiochemical engineering journal · 2013Article
- How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping population.BMC molecular biology · 2013Article
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- BMP and TGFbeta pathways in human central chondrosarcoma: enhanced endoglin and Smad 1 signaling in high grade tumors.BMC cancer · 2012Article
- The Control of Stress Induced Type I Diabetes Mellitus in Humans through the Hepatic Synthesis of Insulin by the Stimulation of Nitric Oxide Production.International journal of biomedical science : IJBS · 2012Article
- Expression of telomeres in astrocytoma WHO grade 2 to 4: TERRA level correlates with telomere length, telomerase activity, and advanced clinical grade.Translational oncology · 2012Article
- RNA integrity in post-mortem samples: influencing parameters and implications on RT-qPCR assays.International journal of legal medicine · 2011Article
- Genome-wide analysis of Ollier disease: Is it all in the genes?Orphanet journal of rare diseases · 2011Article
- The appearance of dermcidin isoform 2, a novel platelet aggregating agent in the circulation in acute myocardial infarction that inhibits insulin synthesis and the restoration by acetyl salicylic acid of its effects.Journal of thrombosis and thrombolysis · 2011Article
- Acetyl salicylic acid (aspirin) improves synthesis of maspin and lowers incidence of metastasis in breast cancer patients [corrected].Cancer science · 2010Article
- The glucose-induced synthesis of insulin in liver.Endocrine · 2010Article
- Comparison of commercial RNA extraction kits for preparation of DNA-free total RNA from Salmonella cells.BMC research notes · 2010Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsHigh quality RNA isolation from cartilaginous tissue is considered difficult because of relatively low cellularity and the abundance of extracellular matrix rich in glycosaminoglycans and collagens. Given the growing interest and technical possibilities to study RNA expression at a high throughput level, research on tissue with these characteristics is hampered by the lack of an efficient method for obtaining sufficient amounts of high quality RNA.
methodsThis paper presents a robust protocol combining two RNA isolation procedures, based on a combination of Trizol and RNA specific columns, which has been developed to obtain high molecular weight RNA from fresh frozen and stored tissue of normal cartilage and cartilaginous tumours. Using this method, RNA was isolated from normal cartilage, peripheral chondrosarcoma, and central chondrosarcoma.
resultsThe yields ranged from 0.1 to 0.5 microg RNA/mg tissue. RNA isolated with this method was stable and of high molecular weight. RNA samples from normal cartilage and from two chondrosarcomas isolated using this method were applied successfully in cDNA microarray experiments. The number of genes that give interpretable results was in the range of what would be expected from microarray results obtained on chondrosarcoma cell line RNA. Signal to noise ratios were good and differential expression between tumour and normal cartilage was detectable for a large number of genes.
conclusionWith this newly developed isolation method, high quality RNA can be obtained from low cellular tissue with a high extracellular matrix component. These procedures can also be applied to other tumour material.
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