ArticlePlant molecular biology2001
Tissue-specific and developmental-specific expression of an Arabidopsis thaliana gene encoding the lipoamide dehydrogenase component of the plastid pyruvate dehydrogenase complex.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content.The Plant cell · 2025Article
- Comparative transcriptomic analysis provides insights into the genetic networks regulating oil differential production in oil crops.BMC biology · 2024Article
- Cassava root membrane proteome reveals activities during storage root maturation.Journal of plant research · 2016Article
- Bidirectional promoters in seed development and related hormone/stress responses.BMC plant biology · 2013Article
- Acyl-lipid metabolism.The arabidopsis book · 2013Article
- Localized hypermutation and associated gene losses in legume chloroplast genomes.Genome research · 2010Article
- Disruption of ptLPD1 or ptLPD2, genes that encode isoforms of the plastidial lipoamide dehydrogenase, confers arsenate hypersensitivity in Arabidopsis.Plant physiology · 2010Article
- Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering.Molecular & cellular proteomics : MCP · 2010Article
- Acyl-lipid metabolism.The arabidopsis book · 2010Article
- Mitochondrial biogenesis and function in Arabidopsis.The arabidopsis book · 2008Article
- Storage reserve accumulation in Arabidopsis: metabolic and developmental control of seed filling.The arabidopsis book · 2008Article
- Mitochondrial acyl carrier proteins in Arabidopsis thaliana are predominantly soluble matrix proteins and none can be confirmed as subunits of respiratory Complex I.Plant molecular biology · 2007Article
- Proteins of the glycine decarboxylase complex in the hydrogenosome of Trichomonas vaginalis.Eukaryotic cell · 2006Article
- Disruption of plE2, the gene for the E2 subunit of the plastid pyruvate dehydrogenase complex, in Arabidopsis causes an early embryo lethal phenotype.Plant molecular biology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe an Arabidopsis thaliana gene, ptlpd2, which codes for a protein with high amino acid similarity to lipoamide dehydrogenases (LPDs) from diverse species. Ptlpd2 codes for a precursor protein possessing an N-terminal extension predicted to be a plastid-targeting signal. Expression of the ptlpd2 cDNA in Escherichia coli showed the encoded protein possessed the predicted LPD activity. PTLPD2 protein, synthesized in vitro, was efficiently imported into isolated chloroplasts of Pisum sativum and shown to be located in the stroma. In addition, fusion proteins containing the predicted transit peptide of PTLPD2 or the entire protein fused at the N-terminus with the green fluorescent protein (GFP), showed accumulation in vivo in chloroplasts but not in mitochondria of A. thaliana. Expression of ptlpd2 was investigated by introducing ptlpd2 promoter-beta-glucuronidase (GUS) gene fusions into Nicotiana tabacum. GUS expression was observed in seeds, flowers, root tips and young leaves. GUS activity was highest in mature seeds, decreased on germination and increased again in young leaves. Expression was also found to be temporally regulated in pollen grains where it was highest in mature grains at dehiscence. Database searches on ptlpd2 sequences identified a second A. thaliana gene encoding a putative plastidial LPD and two genes encoding proteins with high similarity to the mitochondrial LPD of P. sativum.
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