ArticlePlant molecular biology2001
Dual targeting properties of the N-terminal signal sequence of Arabidopsis thaliana THI1 protein to mitochondria and chloroplasts.
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 82 citations in OpenAlex.
- ZmTH1 Is Vital for Healthy Plant Growth and Promotes Cold/Drought Tolerance by Regulating Thiamin Diphosphate-Dependent Metabolisms in Maize.Plant biotechnology journal · 2026Article
- The transgenic Vip3A poplar plant confers high resistance against Hyphantria cunea Drury.Transgenic research · 2026Article
- THI1 Gene Evolutionary Trends: A Comprehensive Plant-Focused Assessment via Data Mining and Large-Scale Analysis.Genome biology and evolution · 2024Article
- TaTHI2 interacts with CaPlant biotechnology journal · 2024Article
- Clock and riboswitch control ofiScience · 2023Article
- Functional and comparative analysis ofPeerJ · 2023Article
- Article
- Rather rule than exception? How to evaluate the relevance of dual protein targeting to mitochondria and chloroplasts.Photosynthesis research · 2018Review
- Pathway Editing Targets for Thiamine Biofortification in Rice Grains.Frontiers in plant science · 2018Review
- Vitamin B1 diversity and characterization of biosynthesis genes in cassava.Journal of experimental botany · 2017Article
- THI1, a Thiamine Thiazole Synthase, Interacts with Ca2+-Dependent Protein Kinase CPK33 and Modulates the S-Type Anion Channels and Stomatal Closure in Arabidopsis.Plant physiology · 2016Article
- Cassava root membrane proteome reveals activities during storage root maturation.Journal of plant research · 2016Article
- Examining strategies to facilitate vitamin B1 biofortification of plants by genetic engineering.Frontiers in plant science · 2013Article
- Article
- Development of insect-resistant transgenic cotton with chimeric TVip3A* accumulating in chloroplasts.Transgenic research · 2011Article
- Physiological and molecular changes in Oryza meridionalis Ng., a heat-tolerant species of wild rice.Journal of experimental botany · 2010Article
- Mitochondrial trafficking of APP and alpha synuclein: Relevance to mitochondrial dysfunction in Alzheimer's and Parkinson's diseases.Biochimica et biophysica acta · 2010Review
- Experimental analysis of the rice mitochondrial proteome, its biogenesis, and heterogeneity.Plant physiology · 2009Article
- Efficient mitochondrial targeting relies on co-operation of multiple protein signals in plants.Journal of experimental botany · 2009Article
- Biochemical and genomic characterization of terpene synthases in Magnolia grandiflora.Plant physiology · 2008Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
thi1 has been recently isolated from Arabidopsis thaliana and is probably involved in both thiamine biosynthesis and as protection of organellar DNA from damage. Studies of thiamine biosynthesis in plants suggests a plastid location for the pathway, which is in agreement with the predicted THI1 N-terminal chloroplastic transit peptide (TP). On the other hand, thiamine is synthesized in mitochondria in yeast cells. Interestingly, A. thaliana thi1 cDNA complements a yeast strain disrupted for the homologous gene. Analysis of THI1 amino acid sequence revealed the presence of a putative amphiphilic alpha-helix, which is typical for mitochondrial presequences, located downstream of the chloroplast transit peptide. To define the putative role of the two predicted targeting sequences in tandem, we produced two chimeric genes encompassing the chloroplastic THI1 TP and either 4 or 27 (including the putative mitochondrial presequence) N-terminal residues of the mature THI1, both linked to the reporter (gusA) gene. Analysis of GUS distribution in subcellular fractions of transgenic plants revealed that in the construct retaining only 4 residues of mature THI1, GUS was found in the chloroplastic fraction. Extension of the THI1 transit peptide to 27 residues of the mature protein allowed import and processing of GUS into both mitochondria and chloroplasts. Direct analysis by immunogold-labeling with an anti-THI1 polyclonal antibody identified THI1 in both organelles in Arabidopsis. We also provide evidence that the precursors of both organellar isoforms are encoded by a single nuclear transcript. Thus, THI1 is targeted simultaneously to mitochondria and chloroplasts by a post transcriptional mechanism.
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