ArticleNucleic acids research2001
Promiscuous patching of broken chromosomes in mammalian cells with extrachromosomal DNA.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 58 citations in OpenAlex.
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- Massive invasion of organellar DNA drives nuclear genome evolution inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Targeted amplification of a sequence of interest in artificial chromosome in mammalian cells.Nucleic acids research · 2019Article
- Stress-inducible NHEJ in bacteria: function in DNA repair and acquisition of heterologous DNA.Nucleic acids research · 2019Article
- Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes.Nature communications · 2016Article
- Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor delivery.Nucleic acids research · 2016Article
- Genome Engineering with TALE and CRISPR Systems in Neuroscience.Frontiers in genetics · 2016Review
- Homology-driven genome editing in hematopoietic stem and progenitor cells using ZFN mRNA and AAV6 donors.Nature biotechnology · 2015Article
- Making designer mutants in model organisms.Development (Cambridge, England) · 2014Review
- TALEN-mediated genetic tailoring as a tool to analyze the function of acquired mutations in multiple myeloma cells.Blood cancer journal · 2014Article
- Whole-genome sequencing identifies genomic heterogeneity at a nucleotide and chromosomal level in bladder cancer.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Replicative mechanisms for CNV formation are error prone.Nature genetics · 2013Article
- Article
- Cytoplasmic organelle DNA preferentially inserts into open chromatin.Genome biology and evolution · 2013Article
- An unbiased genome-wide analysis of zinc-finger nuclease specificity.Nature biotechnology · 2011Article
- Directed networks reveal genomic barriers and DNA repair bypasses to lateral gene transfer among prokaryotes.Genome research · 2011Article
- Intron creation and DNA repair.Cellular and molecular life sciences : CMLS · 2011Review
- Synthesis-dependent microhomology-mediated end joining accounts for multiple types of repair junctions.Nucleic acids research · 2010Article
- Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology.Nucleic acids research · 2010Article
- Analysis of illegitimate genomic integration mediated by zinc-finger nucleases: implications for specificity of targeted gene correction.BMC molecular biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
To study double-strand break (DSB)-induced mutations in mammalian chromosomes, we stably transfected thymidine kinase (tk)-deficient mouse fibroblasts with a DNA substrate containing a recognition site for yeast endonuclease I-SceI embedded within a functional tk gene. Cells were then electroporated with a plasmid expressing endonuclease I-SceI to induce a DSB, and clones that had lost tk function were selected. In a previous study of DSB-induced tk-deficient clones, we found that approximately 8% of recovered tk mutations involved the capture of one or more DNA fragments at the DSB site. Almost half of the DNA capture events involved the I-SceI expression plasmid, and several events involved retrotransposable elements. To learn whether only certain DNA sequences or motifs are efficiently captured, in the current work we electroporated an I-SceI expression plasmid along with HaeIII fragments of φX174 genomic DNA. We report that 18 out of 132 tk-deficient clones recovered had captured DNA fragments, and 14 DNA capture events involved one or more fragments of φX174 DNA. Microhomology existed at most junctions between φX174 DNA and genomic sequences. Our work suggests that virtually any extrachromosomal DNA molecule may be recruited for the patching of DSBs in a mammalian genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.