Evidence map›Paper›PMID 11574599›Full record

ArticleJournal of clinical microbiology2001

PCR for specific detection of H7 flagellar variant of fliC among extraintestinal pathogenic Escherichia coli.

J R Johnson, A L Stell

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

J R JohnsonMedical Service, VA Medical Center, and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55417, USA. johns007@tc.umn.edu
A L Stell

Funding

VIRULENCE OF A LINEAGE OF UROPATHOGENIC ESCHERICHIA COLIR01DK047504 · NIDDK · UNIVERSITY OF MINNESOTA TWIN CITIES · PI JOHNSON, JAMES R · 1993 to 2000
$155k
NIDDK NIH HHS DK-47504
6 · The paper itself

Abstract

A newly developed PCR-based assay for the H7 variant of the Escherichia coli flagellin gene, fliC, was 100% sensitive and specific in comparison with serology and probe hybridization. It revealed broad conservation of the H7 fliC variant among phylogenetically diverse lineages of extraintestinal pathogenic E. coli (ExPEC) and superseded serotyping for certain isolates with ambiguous or non-H7 serotyping results. The H7 primers functioned well when incorporated into a multiplex PCR assay for diverse virulence-associated genes of ExPEC.

Indexed as

Genetic VariationBacterial Typing TechniquesDNA PrimersEscherichia coli InfectionsEscherichia coli O157FlagellinHumansPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSensitivity and SpecificitySerotypingSpecies SpecificityDNA PrimersFlagellin

Identifiers

PMID11574599
PMCPMC88415

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.