ArticleJournal of clinical microbiology2001
Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
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Who cites it
84 citing papers in PubMed.
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- Molecular identification of ligninolytic fungi from West Sumatra, Indonesia, using the Internal Transcribed Spacer (ITS) region.Journal of advanced veterinary and animal research · 2026Article
- Variations in plant's cry for help evidenced by modifications of rice root microbiota induced by blast or brown spot diseases.Environmental microbiome · 2025Article
- Environmental Yeast Abundance and Diversity Assessment in Recreation Areas of Bangkok, Thailand.Environmental microbiology reports · 2025Article
- Overexpression of efflux pump and biofilm associated genes in itraconazole resistant Candida albicans isolates causing onychomycosis.Scientific reports · 2025Article
- Fungi in the Chilean Altiplano: Analyses of Diversity and Yeasts with Applied Enzymatic Potential.Journal of fungi (Basel, Switzerland) · 2025Article
- Discrimination of Clinical and Food-DerivedPathogens (Basel, Switzerland) · 2025Article
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- Long-term biogas slurry fertilization alters rhizosphere soil metabolite profiles and microbial communities in poplar plantations.Frontiers in microbiology · 2025Article
- Endophytic fungi from Cissus quadrangularis plant a promising source of bioactive compounds.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Endophthalmitis patients in Makassar City: molecular identification of pathogenic fungal profile.BMC infectious diseases · 2024Observational
- Diversity Patterns and Drivers of Soil Bacterial and Fungal Communities in a Muddy Coastal Wetland of China.Journal of fungi (Basel, Switzerland) · 2024Article
- Characteristic of new Phaffia rhodozyma yeast strains isolated from birch slime fluxes in Poland.Archives of microbiology · 2024Article
- Granulomatous dermatitis caused byJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- The indigenous microbial diversity involved in the spontaneous fermentation of red dragon fruit (Heliyon · 2023Article
- The Variations of Microbial Diversity and Community Structure Along Different Stream Orders in Wuyi Mountains.Microbial ecology · 2023Article
24 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
Multiplex PCR amplification followed by either agarose gel electrophoresis (PCR-AGE) or microchip electrophoresis (PCR-ME) was used to test a total of 120 fungal strains. The internal transcribed spacer 1 (ITS1) and ITS2 regions and the 5.8S ribosomal DNA (rDNA) region of the fungi were amplified by using universal primers ITS1 and ITS4. The ITS2 region was simultaneously amplified by using universal primers ITS3 and ITS4. Since Trichosporon asahi and T. asteroides showed similar lengths for two amplicons, 29 different gel patterns were demonstrated for 30 yeast species tested on the basis of differences in the lengths of one or two amplicons. Of 75 yeast isolates from clinical materials, 5 isolates (6.8%) which were incompletely identified or not identified by the phenotypic method were identified with our PCR-based method (2 isolates as Candida guilliermondii, 2 as C. krusei, and 1 as C. zeylanoides). No differences in discriminating power or sensitivity were observed between the PCR-AGE method and the PCR-ME method. These methods, prospectively applied to 24 yeast-positive blood culture bottles (16 patients), resulted in the correct detection of 24 yeast strains. In conclusion, multiplex PCR followed by electrophoresis seems to be a promising tool for the rapid identification of common and uncommon yeast strains from culture colonies and from yeast-positive blood culture bottles (5.5 h for the PCR-AGE method and 3 h for the PCR-ME method).
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