ArticleJournal of clinical microbiology2001
Rapid identification of dimorphic and yeast-like fungal pathogens using specific DNA probes.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 149 citations in OpenAlex.
- Accuracy of rapid diagnosis of Talaromyces marneffei: A systematic review and meta-analysis.PloS one · 2018Pooled it
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- A Novel One Health Approach concerning Yeast Present in the Oral Microbiome of the Endangered Rio Skate (Microorganisms · 2023Article
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- Colonized patients byFrontiers in cellular and infection microbiology · 2023Article
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- Diagnosis of histoplasmosis: current status and perspectives.Applied microbiology and biotechnology · 2021Review
- Long-Reads-Based Metagenomics in Clinical Diagnosis With a Special Focus on Fungal Infections.Frontiers in microbiology · 2021Review
- Metagenomics for neurological infections - expanding our imagination.Nature reviews. Neurology · 2020Review
- Frequency Distribution of Keratinophilic Dermatophyte Fungi from the Soil of Different Zones in Isfahan Using Morphological and Molecular Methods.Advanced biomedical research · 2019Article
- A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses.Memorias do Instituto Oswaldo Cruz · 2019Article
- Diagnostic Testing of Neurologic Infections.Neurologic clinics · 2018Review
- Fungemia associated with Schizophyllum commune in Brazil.PLoS neglected tropical diseases · 2017Article
- Genomic characterization of two novel pathogenic avipoxviruses isolated from pacific shearwaters (Ardenna spp.).BMC genomics · 2017Article
- Non-Invasive Methods to Diagnose Fungal Infections in Pediatric Patients with Hematologic Disorders.Jundishapur journal of microbiology · 2016Article
- Candida identification: a journey from conventional to molecular methods in medical mycology.World journal of microbiology & biotechnology · 2014Review
- The emerging world of the fungal microbiome.Trends in microbiology · 2013Review
- Development of specific sequence-characterized amplified region markers for detecting Histoplasma capsulatum in clinical and environmental samples.Journal of clinical microbiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Specific oligonucleotide probes were developed to identify medically important fungi that display yeast-like morphology in vivo. Universal fungal primers ITS1 and ITS4, directed to the conserved regions of ribosomal DNA, were used to amplify DNA from Histoplasma capsulatum, Blastomyces dermatitidis, Coccidioides immitis, Paracoccidioides brasiliensis, Penicillium marneffei, Sporothrix schenckii, Cryptococcus neoformans, five Candida species, and Pneumocystis carinii. Specific oligonucleotide probes to identify these fungi, as well as a probe to detect all dimorphic, systemic pathogens, were developed. PCR amplicons were detected colorimetrically in an enzyme immunoassay format. The dimorphic probe hybridized with DNA from H. capsulatum, B. dermatitidis, C. immitis, P. brasiliensis, and P. marneffei but not with DNA from nondimorphic fungi. Specific probes for H. capsulatum, B. dermatitidis, C. immitis, P. brasiliensis, P. marneffei, S. schenckii, C. neoformans, and P. carinii hybridized with homologous but not heterologous DNA. Minor cross-reactivity was observed for the B. dermititidis probe used against C. immitis DNA and for the H. capsulatum probe used against Candida albicans DNA. However, the C. immitis probe did not cross-react with B. dermititidis DNA, nor did the dimorphic probe hybridize with C. albicans DNA. Therefore, these fungi could be differentiated by a process of elimination. In conclusion, probes developed to yeast-like pathogens were found to be highly specific and should prove to be useful in differentiating these organisms in the clinical setting.
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