ArticleJournal of clinical microbiology2001
Rapid identification of yeasts in positive blood cultures by a multiplex PCR method.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 119 citations in OpenAlex.
- Effects of Partial Pulp Removal on Microbial Dynamics and Metabolite Profiles During On-Farm Cocoa Fermentation.Foods (Basel, Switzerland) · 2026Article
- Comparative genotypic analysis of Candida krusei and Candida parapsilosis isolates from human and animal clinical samples through MLVA and RAPD-PCR profiling.GMS hygiene and infection control · 2026Article
- Detection and Molecular Identification of EightMicroorganisms · 2022Article
- The Characterization of Microbiome and Interactions on Weathered Rocks in a Subsurface Karst Cave, Central China.Frontiers in microbiology · 2022Article
- Truffle Microbiome Is Driven by Fruit Body Compartmentalization Rather than Soils Conditioned by Different Host Trees.mSphere · 2021Article
- Distinct Compartmentalization of Microbial Community and Potential Metabolic Function in the Fruiting Body ofJournal of fungi (Basel, Switzerland) · 2021Article
- Changes in Digestive Microbiota, Rumen Fermentations and Oxidative Stress around Parturition Are Alleviated by Live Yeast Feed Supplementation to Gestating Ewes.Journal of fungi (Basel, Switzerland) · 2021Article
- Prevalence and antifungal drug resistance of nosocomialCurrent medical mycology · 2021Article
- Microbiome Community Structure and Functional Gene Partitioning in Different Micro-Niches Within a Sporocarp-Forming Fungus.Frontiers in microbiology · 2021Article
- Identification of Mycoses in Developing Countries.Journal of fungi (Basel, Switzerland) · 2019Review
- Mosquito-fungus interactions and antifungal immunity.Insect biochemistry and molecular biology · 2019Review
- Autoreactive T Cells and Chronic Fungal Infection Drive Esophageal Carcinogenesis.Cell host & microbe · 2017Article
- Candida identification: a journey from conventional to molecular methods in medical mycology.World journal of microbiology & biotechnology · 2014Review
- A quick and low-cost PCR-based assay for Candida spp. identification in positive blood culture bottles.BMC infectious diseases · 2013Article
- Identification and molecular analysis of pathogenic yeasts in droppings of domestic pigeons in Beijing, China.Mycopathologia · 2012Article
- Insights into Candida tropicalis nosocomial infections and virulence factors.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Review
- Identification of Candida species associated with vulvovaginal candidiasis by multiplex PCR.Infectious diseases in obstetrics and gynecology · 2012Article
- Rapid identification of allergenic and pathogenic molds in environmental air by an oligonucleotide array.BMC infectious diseases · 2011Article
- A new type of DNA polymorphism identified in the species-specific DNA region originating from the Candida albicans mitochondrial genome.Current microbiology · 2009Article
- Simple method to accurately differentiate Candida albicans isolates concurrently using polymorphic patterns of PCR-amplified, species-specific nuclear and mitochondrial targets.Current microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yeasts are emerging as important etiological agents of nosocomial bloodstream infections. A multiplex PCR method was developed to rapidly identify clinically important yeasts that cause fungemia. The method amplified the internal transcribed spacer 1 (ITS1) region between the 18S and 5.8S rRNA genes and a specific DNA fragment within the ITS2 region of Candida albicans. With this method, C. albicans produced two amplicons, whereas other species produced only one. Through sequence analysis, the precise lengths of the PCR products were found to be as follows: C. glabrata (482 or 483 bp), C. guilliermondii (248 bp), C. parapsilosis (229 bp), C. albicans (218 or 219 and 110 bp), C. tropicalis (218 bp), Cryptococcus neoformans (201 bp), and C. krusei (182 bp). The PCR products could be effectively separated by disk polyacrylamide gel electrophoresis. The method was used to test 249 positive blood cultures (255 isolates), from which the following species (strain number) were isolated: C. albicans (128), C. tropicalis (51), C. glabrata (28), C. parapsilosis (23), C. neoformans (9), C. krusei (5), C. guilliermondii (3), and other, minor species (8). The test sensitivity of the method was 96.9% (247 of 255 isolates). The eight minor species were either misidentified (one strain) or not identified (seven strains). From the time at which a positive bottle was found, the multiplex PCR could be completed within 8 h; the present method is simpler than any previously reported molecular method for the identification of blood yeasts.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.