ArticleJournal of clinical microbiology2001
Sensitive and specific method for rapid identification of Streptococcus pneumoniae using real-time fluorescence PCR.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
59 citing papers in PubMed, 1 synthesis or guideline pooled it, 154 citations in OpenAlex.
- PCR using blood for diagnosis of invasive pneumococcal disease: systematic review and meta-analysis.Journal of clinical microbiology · 2010Pooled it
- A three-step diagnosis of pediatric pneumonia at the emergency department using clinical predictors, C-reactive protein, and pneumococcal PCR.European journal of pediatrics · 2017Trial
- Streptococcus pneumoniae from Palestinian nasopharyngeal carriers: serotype distribution and antimicrobial resistance.PloS one · 2013Trial
- Genetic relatedness between pneumococcal populations originating from the nasopharynx, adenoid, and tympanic cavity of children with otitis media.Journal of clinical microbiology · 2005Trial
- No culture? No problem: Clinical utility and pitfalls of non-culture diagnostics for pneumococcal parapneumonic effusions.Frontiers in medicine · 2026Review
- A DAMP-Based Assay for Rapid and Affordable Diagnosis of Bacterial Meningitis Agents:International journal of molecular sciences · 2024Article
- Serotype epidemiology and antibiotic resistance of pneumococcal isolates colonizing infants in Botswana (2016-2019).PloS one · 2024Article
- Development of a diagnostic assay by three-tube multiplex real-time PCR for simultaneous detection of nine microorganisms causing acute respiratory infections.Scientific reports · 2022Article
- Non-diphtheriae Corynebacterium species are associated with decreased risk of pneumococcal colonization during infancy.The ISME journal · 2022Article
- Pneumocystis jirovecii among patients with cystic fibrosis and their household members.Medical mycology · 2021Observational
- Assessment of nasopharyngeal Streptococcus pneumoniae colonization does not permit discrimination between Canadian children with viral and bacterial respiratory infection: a matched-cohort cross-sectional study.BMC infectious diseases · 2021Article
- Relationships among streptococci from the mitis group, misidentified as Streptococcus pneumoniae.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Strategy using a new antigenic test for rapid diagnosis of Streptococcus pneumoniae infection in respiratory samples from children consulting at hospital.BMC microbiology · 2020Article
- Fully Automated Molecular Diagnostic System "Simprova" for Simultaneous Testing of Multiple Items.Scientific reports · 2020Article
- Performance of the Biomark HD real-time qPCR System (Fluidigm) for the detection of nasopharyngeal bacterial pathogens and Streptococcus pneumoniae typing.Scientific reports · 2019Article
- Feasibility of a quantitative polymerase chain reaction assay for diagnosing pneumococcal pneumonia using oropharyngeal swabs.Molecular biology reports · 2019Article
- Human Respiratory Syncytial Virus and Streptococcus pneumoniae Infection in Wild Bonobos.EcoHealth · 2018Article
- Evidence for Human Streptococcus pneumoniae in wild and captive chimpanzees: A potential threat to wild populations.Scientific reports · 2017Article
- Streptococcus pneumoniae antimicrobial resistance decreased in the Helsinki Metropolitan Area after routine 10-valent pneumococcal conjugate vaccination of infants in Finland.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Article
- Surveillance of Australian Hajj pilgrims for carriage of potentially pathogenic bacteria: Data from two pilot studies.World journal of clinical cases · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular surveillance of pathogens has shown the need for rapid and dependable methods for the identification of organisms of clinical and epidemiological importance. As the leading cause of community-acquired pneumonia, Streptococcus pneumoniae was used as a model organism to develop and refine a real-time fluorescence PCR assay and enhanced DNA purification method. Seventy clinical isolates of S. pneumoniae, verified by latex agglutination, were screened against 26 negative control clinical isolates employing a TaqMan assay on a thermocycler (LightCycler). The probe, constructed from the lytA gene, correctly detected all S. pneumoniae genomes without cross-reaction to negative controls. The speed and ease of this approach will make it adaptable to identification of many bacterial pathogens and provide potential for adaptation to direct detection from patient specimens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.