ArticleGenome biology2001
Reassignment of specificities of two cap methyltransferase domains in the reovirus lambda 2 protein.
Article in Genome biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- Characterization of inner capsid σA protein as a virulence factor of the pteropine orthoreovirus.PLoS pathogens · 2026Article
- A universal fluorescence polarization high throughput screening assay to target the SAM-binding sites of SARS-CoV-2 and other viral methyltransferases.Emerging microbes & infections · 2023Article
- CMTr mediated 2'-RNA (New York, N.Y.) · 2022Article
- Methyltransferases ofBiomolecules · 2022Article
- Crystal structure of SARS-CoV-2 nsp10-nsp16 in complex with small molecule inhibitors, SS148 and WZ16.Protein science : a publication of the Protein Society · 2022Article
- Reovirus Activated Cell Death Pathways.Cells · 2022Review
- Enzymatic Assays to Explore Viral mRNA Capping Machinery.Chembiochem : a European journal of chemical biology · 2021Review
- Captivating Perplexities ofViruses · 2021Review
- How Many Mammalian Reovirus Proteins are involved in the Control of the Interferon Response?Pathogens (Basel, Switzerland) · 2019Review
- Synthesis and Translation of Viral mRNA in Reovirus-Infected Cells: Progress and Remaining Questions.Viruses · 2018Review
- African Swine Fever Virus NP868R Capping Enzyme Promotes Reovirus Rescue during Reverse Genetics by Promoting Reovirus Protein Expression, Virion Assembly, and RNA Incorporation into Infectious Virions.Journal of virology · 2017Article
- Loss of a Conserved tRNA Anticodon Modification Perturbs Plant Immunity.PLoS genetics · 2015Article
- A single amino acid substitution in the mRNA capping enzyme λ2 of a mammalian orthoreovirus mutant increases interferon sensitivity.Virology · 2015Article
- Review
- Insights into RNA synthesis, capping, and proofreading mechanisms of SARS-coronavirus.Virus research · 2014Review
- Predicted structure and domain organization of rotavirus capping enzyme and innate immune antagonist VP3.Journal of virology · 2014Article
- Identification of the active sites in the methyltransferases of a transcribing dsRNA virus.Journal of molecular biology · 2014Article
- RNA methyltransferases involved in 5' cap biosynthesis.RNA biology · 2014Review
- Conventional and unconventional mechanisms for capping viral mRNA.Nature reviews. Microbiology · 2011Review
- Bluetongue virus: dissection of the polymerase complex.The Journal of general virology · 2008Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe reovirus lambda2 protein catalyzes mRNA capping, that is, addition of a guanosine to the 5' end of each transcript in a 5'-to-5' orientation, as well as transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to the N7 atom of the added guanosyl moiety and subsequently to the ribose 2'-O atom of the first template-encoded nucleotide. The structure of the human reovirus core has been solved at 3.6 A resolution, revealing a series of domains that include a putative guanylyltransferase domain and two putative methyltransferase (MTase) domains. It has been suggested that the order of domains in the lambda2 protein corresponds to the order of reactions in the pathway and that the m7G (cap 0) and the 2'-O-ribose (cap 1) MTase activities may be exerted by the MTase 1 and the MTase 2 domains, respectively.
resultsWe show that the reovirus MTase 1 domain shares a putative active site with the structurally characterized 2'-O-ribose MTases, including vaccinia virus cap 1 MTase, whereas the MTase 2 domain is structurally similar to glycine N-MTase.
conclusionsOn the basis of our analysis of the structural details we propose that the previously suggested functional assignments of the MTase 1 and MTase 2 domains should be swapped.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.