ArticleOncogene2001
VEGF(165) requires extracellular matrix components to induce mitogenic effects and migratory response in breast cancer cells.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 60 citations in OpenAlex.
- The role of heparins and nano-heparins as therapeutic tool in breast cancer.Glycoconjugate journal · 2017Review
- Interaction of a standardized mistletoe (Viscum album) preparation with antitumor effects of Trastuzumab in vitro.BMC complementary and alternative medicine · 2016Article
- Engineering a High-Throughput 3-D In Vitro Glioblastoma Model.IEEE journal of translational engineering in health and medicine · 2015Article
- Article
- The Methanol Extract of Angelica sinensis Induces Cell Apoptosis and Suppresses Tumor Growth in Human Malignant Brain Tumors.Evidence-based complementary and alternative medicine : eCAM · 2013Article
- Development and fibronectin signaling requirements of the zebrafish interrenal vessel.PloS one · 2012Article
- Accreta complicating complete placenta previa is characterized by reduced systemic levels of vascular endothelial growth factor and by epithelial-to-mesenchymal transition of the invasive trophoblast.American journal of obstetrics and gynecology · 2011Article
- Laminin alpha4-null mutant mice develop chronic kidney disease with persistent overexpression of platelet-derived growth factor.The American journal of pathology · 2010Article
- Shorter survival-times following adjuvant endocrine therapy in oestrogen- and progesterone-receptor positive breast cancer overexpressing HER2 and/or with an increased expression of vascular endothelial growth factor.Medical oncology (Northwood, London, England) · 2009Article
- Estrogen-related receptor alpha induces the expression of vascular endothelial growth factor in breast cancer cells.The Journal of steroid biochemistry and molecular biology · 2009Article
- A catalytic role of heparin within the extracellular matrix.The Journal of biological chemistry · 2008Article
- Article
- Overexpression of vascular endothelial growth factor 189 in breast cancer cells leads to delayed tumor uptake with dilated intratumoral vessels.The American journal of pathology · 2008Article
- The role of VEGF receptors in angiogenesis; complex partnerships.Cellular and molecular life sciences : CMLS · 2006Review
- Article
- Calponin h1 suppresses tumor growth of Src-induced transformed 3Y1 cells in association with a decrease in angiogenesis.Japanese journal of cancer research : Gann · 2002Article
- Fibronectin Mechanobiology Regulates Tumorigenesis.Cellular and molecular bioengineeringReview
- Overexpression of VEGF189 in breast cancer cells induces apoptosis via NRP1 under stress conditions.Cell adhesion & migrationArticle
- Autocrine functions of VEGF in breast tumor cells: adhesion, survival, migration and invasion.Cell adhesion & migrationReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The expression of VEGF and the relapse-free survival rate of breast cancer patients are inversely related. While VEGF induces the proliferation and migration of vascular endothelial cells, its function in breast cancer cells is not well studied. We reported previously that fibronectin increased VEGF-dependent migration in breast cancer cells. Since VEGF has an extracellular matrix (ECM)-binding domain and possesses binding affinity for heparin, we sought to determine the effects of VEGF in breast cancer cells and the role of heparin and/or fibronectin in VEGF-induced signaling. Cells grown on plastic were compared to those grown on fibronectin or to those grown on plastic in the presence of heparin, and analysed for intracellular signaling, proliferation and migration in response to VEGF(165). Both heparin and fibronectin enhanced the binding of VEGF to T47D cells. After treatment with VEGF, [(3)H]thymidine incorporation, c-fos induction, and the number of migrating cells were significantly higher ( approximately twofold) in cells grown on fibronectin or in cells grown on plastic in the presence of heparin when compared to those grown on plastic only. Likewise, tyrosine phosphorylation of VEGF receptors, MAPK activity and PI3-kinase activity were all several-fold higher in cells seeded on fibronectin or in the presence of heparin as compared to cells exposed to VEGF alone. VEGF-dependent c-fos induction was found to be regulated through a MAPK-dependent, but PI3-kinase-independent pathway. In contrast, the migration of T47D cells in response to VEGF, in the presence of ECM, was regulated through PI3-kinase. Therefore, VEGF requires ECM components to induce a mitogenic response and cell migration in T47D breast cancer cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.