ArticleApplied and environmental microbiology2001
TaqMan PCR for detection of Vibrio cholerae O1, O139, non-O1, and non-O139 in pure cultures, raw oysters, and synthetic seawater.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 134 citations in OpenAlex.
- Development of a novel strategy to reduce diagnostic errors in real-time polymerase chain reaction using probe-based techniques.Scientific reports · 2024Article
- Direct and Rapid Identification of Vibrio Cholerae Serogroup and Toxigenicity by a Novel Multiplex Real-Time Assay.Pathogens (Basel, Switzerland) · 2022Article
- Foodborne Pathogenic Vibrios: Antimicrobial Resistance.Frontiers in microbiology · 2021Review
- Simultaneous Quantification ofPathogens (Basel, Switzerland) · 2020Article
- Role of Indole Production on Virulence of Vibrio cholerae Using Galleria mellonella Larvae Model.Indian journal of microbiology · 2016Article
- A novel triplex quantitative PCR strategy for quantification of toxigenic and nontoxigenic Vibrio cholerae in aquatic environments.Applied and environmental microbiology · 2015Article
- Article
- Multipurpose assessment for the quantification of Vibrio spp. and total bacteria in fish and seawater using multiplex real-time polymerase chain reaction.Journal of the science of food and agriculture · 2014Article
- Pre-earthquake non-epidemic Vibrio cholerae in Haiti.Journal of infection in developing countries · 2014Article
- Multiplex Real-time Polymerase Chain Reaction Assays for Simultaneous Detection of Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus.Osong public health and research perspectives · 2013Article
- Rapid and sensitive quantification of Vibrio cholerae and Vibrio mimicus cells in water samples by use of catalyzed reporter deposition fluorescence in situ hybridization combined with solid-phase cytometry.Applied and environmental microbiology · 2012Article
- Enhanced detection of Rift Valley fever virus using molecular assays on whole blood samples.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2012Article
- Detection, isolation, and identification of Vibrio cholerae from the environment.Current protocols in microbiology · 2012Article
- Quadruplex real-time PCR assay for detection and identification of Vibrio cholerae O1 and O139 strains and determination of their toxigenic potential.Applied and environmental microbiology · 2009Article
- Simultaneous detection of enteric bacteria from surface waters by QPCR in comparison with conventional bacterial indicators.Environmental monitoring and assessment · 2009Article
- Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loop-mediated isothermal amplification.BMC microbiology · 2008Article
- Vibrio cholerae in the environment: a simple method for reliable identification of the species.Journal of health, population, and nutrition · 2007Article
- Detection of Vibrio cholerae by real-time nucleic acid sequence-based amplification.Applied and environmental microbiology · 2007Article
- Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae.Applied and environmental microbiology · 2006Article
- Real-time PCR detection of Vibrio vulnificus in oysters: comparison of oligonucleotide primers and probes targeting vvhA.Applied and environmental microbiology · 2005Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vibrio cholerae is recognized as a leading human waterborne pathogen. Traditional diagnostic testing for Vibrio is not always reliable, because this bacterium can enter a viable but nonculturable state. Therefore, nucleic acid-based tests have emerged as a useful alternative to traditional enrichment testing. In this article, a TaqMan PCR assay is presented for quantitative detection of V. cholerae in pure cultures, oysters, and synthetic seawater. Primers and probe were designed from the nonclassical hemolysin (hlyA) sequence of V. cholerae strains. This probe was applied to DNA from 60 bacterial strains comprising 21 genera. The TaqMan PCR assay was positive for all of the strains of V. cholerae tested and negative for all other species of Vibrio tested. In addition, none of the other genera tested was amplified with the TaqMan primers and probe used in this study. The results of the TaqMan PCR with raw oysters and spiked with V. cholerae serotypes O1 and O139 were comparable to those of pure cultures. The sensitivity of the assay was in the range of 6 to 8 CFU g(-1) and 10 CFU ml(-1) in spiked raw oyster and synthetic seawater samples, respectively. The total assay could be completed in 3 h. Quantification of the Vibrio cells was linear over at least 6 log units. The TaqMan probe and primer set developed in this study can be used as a rapid screening tool for the presence of V. cholerae in oysters and seawater without prior isolation and characterization of the bacteria by traditional microbiological methods.
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