ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Estrogen and Bcl-2: gene induction and effect of transgene in experimental stroke.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
66 citing papers in PubMed, 3 syntheses or guidelines pooled it, 179 citations in OpenAlex.
- A comprehensive meta-analysis of exogenous estrogen, progesterone, and testosterone in animal models of ischemic and hemorrhagic stroke.Biology of sex differences · 2026Pooled it
- Impact of methodology on estrogens' effects on cerebral ischemia in rats: an updated meta-analysis.BMC neuroscience · 2014Pooled it
- Mechanisms of estrogens' dose-dependent neuroprotective and neurodamaging effects in experimental models of cerebral ischemia.International journal of molecular sciences · 2011Pooled it
- Sex-dependent role of 20-HETE synthesis in outcome from ischemic stroke in rats.Physiological reports · 2026Article
- The Role of Estradiol in Traumatic Brain Injury: Mechanism and Treatment Potential.International journal of molecular sciences · 2020Review
- Article
- Estrogens as neuroprotectants: Estrogenic actions in the context of cognitive aging and brain injury.Progress in neurobiology · 2017Review
- HO1 and Wnt expression is independently regulated in female mice brains following permanent ischemic brain injury.Brain research · 2017Article
- Sex Difference in Oxidative Stress Parameters in Spinal Cord of Rats with Experimental Autoimmune Encephalomyelitis: Relation to Neurological Deficit.Neurochemical research · 2017Article
- Neuroprotective Role of Steroidal Sex Hormones: An Overview.CNS neuroscience & therapeutics · 2016Review
- Administration of low dose estrogen attenuates gliosis and protects neurons in acute spinal cord injury in rats.Journal of neurochemistry · 2016Article
- Estrogen provides neuroprotection against brain edema and blood brain barrier disruption through both estrogen receptors α and β following traumatic brain injury.Iranian journal of basic medical sciences · 2015Article
- Central inflammatory response to experimental stroke is inhibited by a neuroprotective dose of dietary soy.Brain research · 2014Article
- 17β-estradiol and inflammation: implications for ischemic stroke.Aging and disease · 2014Review
- Non-feminizing estrogens: a novel neuroprotective therapy.Molecular and cellular endocrinology · 2014Review
- Effects of high and low 17β-estradiol doses on focal cerebral ischemia: negative results.Scientific reports · 2013Article
- Experimental pediatric arterial ischemic stroke model reveals sex-specific estrogen signaling.Stroke · 2013Article
- Sex differences in brain proteomes of neuron-specific STAT3-null mice after cerebral ischemia/reperfusion.Journal of neurochemistry · 2012Article
- Estradiol protects against hippocampal damage and impairments in fear conditioning resulting from transient global ischemia in mice.Brain research · 2012Article
- Cell surface estrogen receptor alpha is upregulated during subchronic metabolic stress and inhibits neuronal cell degeneration.PloS one · 2012Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Female rodents producing endogenous estrogens are protected from stroke damage in comparison with male counterparts. This natural protection is lost after ovariectomy or reproductive senescence. The aim of this study is to determine whether estrogen reduces early neuronal injury and cell loss after ischemia by increasing the expression of Bcl-2. Male, intact female, ovariectomized, and estrogen-repleted ovariectomized rats were subjected to middle cerebral artery occlusion, and 22 hr later the level and localization of Bcl-2 mRNA and protein were determined. The levels of post-ischemic bcl-2 mRNA and protein were increased exclusively in neurons within the peri-infarct region. Intact females and estrogen-treated castrates demonstrated increased bcl-2 mRNA and protein expression compared with males and estrogen-deficient females, accompanied by a decrease in infarct size. To test the hypothesis that the neuroprotective mechanism of estrogen functions via Bcl-2, we compared ischemic outcome in male, female, and ovariectomized wild-type mice and mice overexpressing Bcl-2 exclusively in neurons. Wild-type female mice sustained smaller infarcts compared with males. Bcl-2 overexpression reduced infarct size in males, but provided no added protection in the female. Moreover, ovariectomy exacerbated infarction in wild-type females, but had no effect in Bcl-2 overexpressors. These data indicate that overexpression of Bcl-2 simulates the protection against ischemic injury conferred by endogenous female sex steroids. We concluded that estrogen rescues neurons after focal cerebral ischemia by increasing the level of Bcl-2 in peri-infarct regions and that estrogen-induced bcl-2 gene expression is an important downstream component of neuronal protection in female stroke.
Indexed as
Identifiers
11567044PMC6762919W2153146414What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.