ArticleJournal of bacteriology2001
uvrA is an acid-inducible gene involved in the adaptive response to low pH in Streptococcus mutans.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 112 citations in OpenAlex.
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- Identification and functional analysis of glutamine transporter inJournal of oral microbiology · 2020Article
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- Acid-resistant genes of oral plaque microbiome from the functional metagenomics.Journal of oral microbiology · 2018Article
- Transcriptome responses of Streptococcus mutans to peroxide stress: identification of novel antioxidant pathways regulated by Spx.Scientific reports · 2017Article
- Acid-adaptive mechanisms of Streptococcus mutans-the more we know, the more we don't.Molecular oral microbiology · 2017Review
- UvrA expression ofJournal of food science and technology · 2017Article
- Analysis of Small RNAs in Streptococcus mutans under Acid Stress-A New Insight for Caries Research.International journal of molecular sciences · 2016Article
- Stress Physiology of Lactic Acid Bacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Trk2 Potassium Transport System in Streptococcus mutans and Its Role in Potassium Homeostasis, Biofilm Formation, and Stress Tolerance.Journal of bacteriology · 2016Article
- Adaptation inFrontiers in microbiology · 2016Review
- Transcriptional profile of glucose-shocked and acid-adapted strains of Streptococcus mutans.Molecular oral microbiology · 2015Article
- Stress tolerance and virulence of insect-pathogenic fungi are determined by environmental conditions during conidial formation.Current genetics · 2015Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pH-inducible acid tolerance response (ATR) is believed to play a major role in acid adaptation and virulence of Streptococcus mutans. To study this phenomenon in S. mutans JH1005, differential display PCR was used to identify and clone 13 cDNA products that had increased expression in response to pH 5.0 compared to that of pH 7.5-grown cells. One of these products, confirmed to be pH inducible by RNA dot blot and reverse transcription-PCR analyses, had 67% identity to a uvrA-UV repair excinuclease gene in Bacillus subtilis. Further sequence analysis of the uvrA homologue using the S. mutans genome database revealed that the complete gene was encoded in an open reading frame (ORF) of 2,829 bp (944 amino acids; 104.67 kDa). Immediately 3' of uvrA was an ORF encoding a putative aminopeptidase gene (pepP). uvrA knockouts were constructed in S. mutans strains JH1005, NG8, and UA159 using allelic-exchange mutagenesis, replacing the entire gene with an erythromycin resistance cassette. As with uvrA mutants in other bacteria, the S. mutans uvrA mutants were extremely sensitive to UV irradiation. The uvrA mutant of S. mutans JH1005 was also more sensitive than the wild type to growth at pH 5.0, showing a 15% reduction in growth rate and a 14% reduction in final resting culture density. Acid-adapted S. mutans JH1005 uvrA mutants were shown to be more resistant to UV irradiation than was the parent but were unable to survive exposure to a killing pH of 3.0. Moreover, agarose gel electrophoretic analysis of chromosomal DNA isolated from uvrA-deficient cells exposed to low pH demonstrated more DNA damage than that for the wild-type strain. Here we suggest that uvrA and the nucleotide excision repair pathway are involved in the repair of acid-induced DNA damage and are associated with successful adaptation of S. mutans to low pH.
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