ArticleJournal of bacteriology2001
GerN, an endospore germination protein of Bacillus cereus, is an Na(+)/H(+)-K(+) antiporter.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Niallia yueguangensis sp. nov., a novel bacterium from maize rhizosphere in saline-alkaline soil enhancing plant salt tolerance.BMC microbiology · 2026Article
- Atypical Serogroup IVb-v1 ofFrontiers in microbiology · 2022Article
- Novel Cesium Resistance Mechanism of Alkaliphilic Bacterium Isolated From Jumping Spider Ground Extract.Frontiers in microbiology · 2022Article
- Salt Tolerance Mechanism and Species Identification of the Plant Rhizosphere Bacterium JYZ-SD2.Current microbiology · 2020Article
- Polar or Charged Residues Located in Four Highly Conserved Motifs Play a Vital Role in the Function or pH Response of a UPF0118 Family NaFrontiers in microbiology · 2020Article
- Broad phylogenetic analysis of cation/proton antiporters reveals transport determinants.Nature communications · 2018Article
- An Uncharacterized Major Facilitator Superfamily Transporter FromFrontiers in microbiology · 2018Article
- Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein FromFrontiers in microbiology · 2018Article
- Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction.BMC microbiology · 2012Article
- A Bacillus flagellar motor that can use both Na+ and K+ as a coupling ion is converted by a single mutation to use only Na+.PloS one · 2012Article
- Conserved and diversified gene families of monovalent cation/h(+) antiporters from algae to flowering plants.Frontiers in plant science · 2012Article
- The halophilic alkalithermophile Natranaerobius thermophilus adapts to multiple environmental extremes using a large repertoire of Na(K)/H antiporters.Molecular microbiology · 2009Article
- GerO, a putative Na+/H+-K+ antiporter, is essential for normal germination of spores of the pathogenic bacterium Clostridium perfringens.Journal of bacteriology · 2009Article
- The Bacillus cereus GerN and GerT protein homologs have distinct roles in spore germination and outgrowth, respectively.Journal of bacteriology · 2008Article
- Three two-component transporters with channel-like properties have monovalent cation/proton antiport activity.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Three putative cation/proton antiporters from the soda lake alkaliphile Alkalimonas amylolytica N10 complement an alkali-sensitive Escherichia coli mutant.Microbiology (Reading, England) · 2007Article
- Cloning of two SOS1 transporters from the seagrass Cymodocea nodosa. SOS1 transporters from Cymodocea and Arabidopsis mediate potassium uptake in bacteria.Plant molecular biology · 2007Article
- Importance of the GP dipeptide of the antiporter motif and other membrane-embedded proline and glycine residues in tetracycline efflux protein Tet(L).Biochemistry · 2005Article
- Halotolerant cyanobacterium Aphanothece halophytica contains NapA-type Na+/H+ antiporters with novel ion specificity that are involved in salt tolerance at alkaline pH.Applied and environmental microbiology · 2005Article
- Expression patterns of a novel AtCHX gene family highlight potential roles in osmotic adjustment and K+ homeostasis in pollen development.Plant physiology · 2004Article
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4 authors.
Funding
Abstract
GerN, a Bacillus cereus spore germination protein, exhibits homology to a widely distributed group of putative cation transporters or channel proteins. GerN complemented the Na(+)-sensitive phenotype of an Escherichia coli mutant that is deficient in Na(+)/H(+) antiport activity (strain KNabc). GerN also reduced the concentration of K(+) required to support growth of an E. coli mutant deficient in K(+) uptake (strain TK2420). In a fluorescence-based assay of everted E. coli KNabc membrane vesicles, GerN exhibited robust Na(+)/H(+) antiport activity, with a K(m) for Na(+) estimated at 1.5 mM at pH 8.0 and 25 mM at pH 7.0. Li(+), but not K(+), served as a substrate. GerN-mediated Na(+)/H(+) antiport was further demonstrated in everted vesicles as energy-dependent accumulation of (22)Na(+). GerN also used K(+) as a coupling ion without completely replacing H(+), as indicated by partial inhibition by K(+) of H(+) uptake into right-side-out vesicles loaded with Na(+). K(+) translocation as part of the antiport was supported by the stimulatory effect of intravesicular K(+) on (22)Na(+) uptake by everted vesicles and the dependence of GerN-mediated (86)Rb(+) efflux on the presence of Na(+) in trans. The inhibitory patterns of protonophore and thiocyanate were most consistent with an electrogenic Na(+)/H(+)-K(+) antiport. GerN-mediated Na(+)/H(+)-K(+) antiport was much more rapid than GerN-mediated Na(+)/H(+) antiport.
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