ArticleJournal of bacteriology2001
Role of NtcB in activation of nitrate assimilation genes in the cyanobacterium Synechocystis sp. strain PCC 6803.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 71 citations in OpenAlex.
- Integrated genomic and phenotypic analysis of an endophytic bacterium reveals biocontrol and plant growth-promoting mechanisms.iScience · 2026Article
- Coordination of Cyanobacterial Nitrate Assimilation and Photosynthesis by a Novel PsbO-Interacting Protein PirN.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- How Small Proteins Adjust the Metabolism of Cyanobacteria Under Stress: The Role of Small Proteins in Cyanobacterial Stress Responses.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Allosteric regulation of nitrate transporter NRT via the signaling protein PII.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- DNA looping mediates cooperative transcription activation.Nature structural & molecular biology · 2024Article
- The primary carbon metabolism in cyanobacteria and its regulation.Frontiers in plant science · 2024Review
- Draft Genome Sequences ofJournal of genomics · 2023Article
- A mechanistic study of the influence of nitrogen and energy availability on the NH4+ sensitivity of nitrogen assimilation in Synechococcus.Journal of experimental botany · 2022Article
- Genetic Responses of Metabolically ActiveMicroorganisms · 2021Article
- Discovery of Euryhaline Phycoerythrobilin-ContainingmSystems · 2020Article
- Impact of RNase E and RNase J on Global mRNA Metabolism in the CyanobacteriumFrontiers in microbiology · 2020Article
- Glycogen Production in Marine Cyanobacterial Strain Synechococcus sp. NKBG 15041c.Marine biotechnology (New York, N.Y.) · 2018Article
- Regulatory components of carbon concentrating mechanisms in aquatic unicellular photosynthetic organisms.Plant cell reports · 2017Review
- The Acceptor Side of Photosystem II Is the Initial Target of Nitrite Stress in Synechocystis sp. Strain PCC 6803.Applied and environmental microbiology · 2017Article
- Functional Diversity of Transcriptional Regulators in the CyanobacteriumFrontiers in microbiology · 2017Article
- Finding approximate gene clusters with Gecko 3.Nucleic acids research · 2016Article
- UpCoT: an integrated pipeline tool for clustering upstream DNA sequences of orthologous genes in prokaryotic genomes.3 Biotech · 2016Article
- Induction of the Nitrate Assimilation nirA Operon and Protein-Protein Interactions in the Maturation of Nitrate and Nitrite Reductases in the Cyanobacterium Anabaena sp. Strain PCC 7120.Journal of bacteriology · 2015Article
- Regulation of CO2 Concentrating Mechanism in Cyanobacteria.Life (Basel, Switzerland) · 2015Review
- Systematic genomic analysis reveals the complementary aerobic and anaerobic respiration capacities of the human gut microbiota.Frontiers in microbiology · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Synechocystis sp. strain PCC 6803, the genes encoding the proteins involved in nitrate assimilation are organized into two transcription units, nrtABCD-narB and nirA, the expression of which was repressed by ammonium and induced by inhibition of ammonium assimilation, suggesting involvement of NtcA in the transcriptional regulation. Under inducing conditions, expression of the two transcription units was enhanced by nitrite, suggesting regulation by NtcB, the nitrite-responsive transcriptional enhancer we previously identified in Synechococcus sp. strain PCC 7942. The slr0395 gene, which encodes a protein 47% identical to Synechococcus NtcB, was identified as the Synechocystis ntcB gene, on the basis of the inability of an slr0395 mutant to rapidly accumulate the transcripts of the nitrate assimilation genes upon induction and to respond to nitrite. While Synechococcus NtcB strictly requires nitrite for its action, Synechocystis NtcB enhanced transcription significantly even in the absence of nitrite. Whereas the Synechococcus ntcB mutant expresses the nitrate assimilation genes to a significant level in an NtcA-dependent manner, the Synechocystis ntcB mutant showed only low-level expression of the nitrate assimilation genes, indicating that NtcA by itself cannot efficiently promote expression of these genes in Synechocystis. Activities of the nitrate assimilation enzymes in the Synechocystis ntcB mutant were consequently low, being 40 to 50% of the wild-type level, and the cells grew on nitrate at a rate approximately threefold lower than that of the wild-type strain. These results showed that the contribution of NtcB to the expression of nitrate assimilation capability varies considerably among different strains of cyanobacteria.
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