ArticleBiophysical journal2001
Bilayer reconstitution of voltage-dependent ion channels using a microfabricated silicon chip.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 133 citations in OpenAlex.
- Two-step formation of multilayered membrane pattern from living cells and probing its nanoscale physicochemical properties via nanopipette.Materials today. Bio · 2026Article
- High bandwidth approaches in nanopore and ion channel recordings - A tutorial review.Analytica chimica acta · 2019Review
- Photolithographic Fabrication of Micro Apertures in Dry Film Polymer Sheets for Channel Recordings in Planar Lipid Bilayers.The Journal of membrane biology · 2019Article
- Electrochemical impedance spectroscopy for black lipid membranes fused with channel protein supported on solid-state nanopore.European biophysics journal : EBJ · 2016Article
- Decreased aperture surface energy enhances electrical, mechanical, and temporal stability of suspended lipid membranes.ACS applied materials & interfaces · 2013Article
- Green fluorescent protein changes the conductance of connexin 43 (Cx43) hemichannels reconstituted in planar lipid bilayers.The Journal of biological chemistry · 2012Article
- Rapid topology probing using fluorescence spectroscopy in planar lipid bilayer: the pore-forming mechanism of the toxin Cry1Aa of Bacillus thuringiensis.The Journal of general physiology · 2010Article
- Microtechnologies for membrane protein studies.Analytical and bioanalytical chemistry · 2008Review
- Detection of the opening of the bundle crossing in KcsA with fluorescence lifetime spectroscopy reveals the existence of two gates for ion conduction.The Journal of general physiology · 2006Article
- Channel activity of OmpF monitored in nano-BLMs.Biophysical journal · 2006Article
- A novel method for patch-clamp automation.Pflugers Archiv : European journal of physiology · 2006Article
- Photocurrents generated by bacteriorhodopsin adsorbed on nano-black lipid membranes.Biophysical journal · 2005Article
- An air-molding technique for fabricating PDMS planar patch-clamp electrodes.Pflugers Archiv : European journal of physiology · 2005Article
- Impedance analysis and single-channel recordings on nano-black lipid membranes based on porous alumina.Biophysical journal · 2004Article
- Whole-cell recording of intracellular pH with silanized and oiled patch-type single or double-barreled microelectrodes.Pflugers Archiv : European journal of physiology · 2003Article
- Microfabricated teflon membranes for low-noise recordings of ion channels in planar lipid bilayers.Biophysical journal · 2003Article
- Regulation of calcium channel activity by lipid domain formation in planar lipid bilayers.Biophysical journal · 2003Article
- Simultaneous optical and electrical recording of single gramicidin channels.Biophysical journal · 2003Article
- Biology on a chip: microfabrication for studying the behavior of cultured cells.Critical reviews in biomedical engineering · 2003Review
- Whole cell patch clamp recording performed on a planar glass chip.Biophysical journal · 2002Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Painted bilayers containing reconstituted ion channels serve as a well defined model system for electrophysiological investigations of channel structure and function. Horizontally oriented bilayers with easy solution access to both sides were obtained by painting a phospholipid:decane mixture across a cylindrical pore etched into a 200-microm thick silicon wafer. Silanization of the SiO(2) layer produced a hydrophobic surface that promoted the adhesion of the lipid mixture. Standard lithographic techniques and anisotropic deep-reactive ion etching were used to create pores with diameters from 50 to 200 microm. The cylindrical structure of the pore in the partition and the surface treatment resulted in stable bilayers. These were used to reconstitute Maxi K channels in the 100- and 200-microm diameter pores. The electrophysiological characteristics of bilayers suspended in microchips were comparable with that of other bilayer preparations. The horizontal orientation and good voltage clamping properties make the microchip bilayer method an excellent system to study the electrical properties of reconstituted membrane proteins simultaneously with optical probes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.