ArticleBiophysical journal2001
Binding of cross-linked glycosylphosphatidylinositol-anchored proteins to discrete actin-associated sites and cholesterol-dependent domains.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 56 citations in OpenAlex.
- Consequences of Heterogeneous Crowding on an Enzymatic Reaction: A Residence Time Monte Carlo Approach.ACS omega · 2019Article
- GPI-anchored protein organization and dynamics at the cell surface.Journal of lipid research · 2016Review
- Plasticity of plasma membrane compartmentalization during plant immune responses.Frontiers in plant science · 2012Article
- Signaling clusters in the cell membrane.Current opinion in cell biology · 2011Review
- Membrane domains and flagellar pocket boundaries are influenced by the cytoskeleton in African trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Activation of T cells by cross-linking Qa-2, the ped gene product, requires Fyn.American journal of reproductive immunology (New York, N.Y. : 1989) · 2007Article
- Single-particle tracking of membrane protein diffusion in a potential: simulation, detection, and application to confined diffusion of CFTR Cl- channels.Biophysical journal · 2007Article
- Dynamic recruitment of phospholipase C gamma at transiently immobilized GPI-anchored receptor clusters induces IP3-Ca2+ signaling: single-molecule tracking study 2.The Journal of cell biology · 2007Article
- Cholesterol dictates the freedom of EGF receptors and HER2 in the plane of the membrane.Biophysical journal · 2005Article
- Rapid hop diffusion of a G-protein-coupled receptor in the plasma membrane as revealed by single-molecule techniques.Biophysical journal · 2005Article
- Dynamics of putative raft-associated proteins at the cell surface.The Journal of cell biology · 2004Article
- Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques.Biophysical journal · 2004Article
- Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4,5-bisphosphate-dependent organization of cell actin.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Translational diffusion of individual class II MHC membrane proteins in cells.Biophysical journal · 2002Article
- Nonpolarized cells selectively sort apical proteins from cell surface to a novel compartment, but lack apical retention mechanisms.Molecular biology of the cell · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanism by which cross-linked glycosylphosphatidylinositol (GPI)-anchored proteins are immobilized has been a mystery because both the binding to a transmembrane protein and attachment to a rigid cytoskeleton are needed. Using laser tweezers surface scanning resistance (SSR) technology, we obtained physical evidence for cross-linked GPI-anchored protein, Qa-2, binding to a transmembrane protein and for diffusion to discrete cytoskeleton attachment sites. At low levels of cross-linking of Qa-2 molecules, the resistance to lateral movement was that expected of monomeric lipid-anchored proteins, and no specific binding to cytoskeleton-attached structures was observed. When aggregates of the GPI-anchored protein, Qa-2, were scanned across plasma membranes, the background resistance was much higher than expected for a GPI-anchored protein alone and submicron domains of even higher resistance were observed (designated as elastic or non-elastic barriers) at a density of 82 (61 elastic and 21 small non-elastic barriers) per 100 microm(2). Elastic barriers involved weak but specific bonds to the actin cytoskeleton (broken by forces of 2 or 4 pN and were removed by cytochalasin D). Small non-elastic barriers (50-100 nm) depended upon membrane cholesterol and were closely correlated with caveolae density. Thus, cross-linked GPI-anchored proteins can diffuse through the membrane in complex with a transmembrane protein and bind weakly to discrete cytoskeleton attachment sites either associated with flexible actin networks or sphingolipid-cholesterol rich microdomains in live cell membranes. Our SSR measurements provide the first description of the physical characteristics of the interactions between rafts and stable membrane structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.