ArticleBiophysical journal2001
Two-dimensional kinetic analysis suggests nonsequential gating of mechanosensitive channels in Xenopus oocytes.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Microscopic mechanism of PIEZO1 activation by pressure-induced membrane stretch.The Journal of general physiology · 2023Article
- Utrophin regulates modal gating of mechanosensitive ion channels in dystrophic skeletal muscle.The Journal of physiology · 2014Article
- Single-channel kinetics of BK (Slo1) channels.Frontiers in physiology · 2014Review
- Single mechanosensitive and Ca²⁺-sensitive channel currents recorded from mouse and human embryonic stem cells.The Journal of membrane biology · 2013Article
- Partial opening and subconductance gating of mechanosensitive ion channels in dystrophic skeletal muscle.The Journal of physiology · 2012Article
- Voltage- and cold-dependent gating of single TRPM8 ion channels.The Journal of general physiology · 2011Article
- Coupling and cooperativity in voltage activation of a limited-state BK channel gating in saturating Ca2+.The Journal of general physiology · 2010Article
- Linking exponential components to kinetic states in Markov models for single-channel gating.The Journal of general physiology · 2008Article
- Twenty odd years of stretch-sensitive channels.Pflugers Archiv : European journal of physiology · 2006Review
- Estimating binding affinities of the nicotinic receptor for low-efficacy ligands using mixtures of agonists and two-dimensional concentration-response relationships.The Journal of general physiology · 2006Article
- Gating reaction mechanisms for NMDA receptor channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Model-based fitting of single-channel dwell-time distributions.Biophysical journal · 2004Article
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3 authors.
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Abstract
Xenopus oocytes express mechanosensitive (MS(XO)) channels that can be studied in excised patches of membrane with the patch-clamp technique. This study examines the steady-state kinetic gating properties of MS(XO) channels using detailed single-channel analysis. The open and closed one-dimensional dwell-time distributions were described by the sums of 2-3 open and 5-7 closed exponential components, respectively, indicating that the channels enter at least 2-3 open and 5-7 closed kinetic states during gating. Dependency plots revealed that the durations of adjacent open and closed intervals were correlated, indicating two or more gateway states in the gating mechanism for MS channels. Maximum likelihood fitting of two-dimensional dwell-time distributions to both generic and specific models was used to examine gating mechanism and rank models. A kinetic scheme with five closed and five open states, in which each closed state could make a direct transition to an open state (two-tiered model) could account for the major features of the single-channel data. Two-tiered models that allowed direct transitions to subconductance open states in addition to the fully open state were also consistent with multiple gateway states. Thus, the gating mechanism of MS(XO) channels differs from the sequential (linear) gating mechanisms considered for MS channels in bacteria, chick skeletal muscle, and Necturus proximal tubule.
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